Sequences from confirmation amplicon


Confirmation sequences for line 665A02 and hit At5g09400 are shown.
Line ID 665A02
Locus At5g09400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2919427
Primers used for PCR ST08 - 8474, show primer details
   
Sequence Name 54-K103548-0022-665-A02C-ST08
GenBank Accession LN508449 [GenBank]
Sequencing primer gene specific primer, ST08: 5'-ATTAAACAGTCAACAGCTCTGGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K103548-0022-665-A02C-ST08
CTTCTCAAATTATGGGCCAGATTTATATACCTGTCCTAAACTGGTTCCTGCTTGCCGTTT
GCCTGGTCGTCGTCTGCTCAATCTCAAGTATCGATGAGATTGGAAATGCATATGGTAATA
GTTGTTTTCTATGCTTCCCTGTCTAGCTTCGGTGATCCGATATGCTTCCCCCGTTTTGAT
TTTCTTTTTCGATTTCCCAGGGATGGCTGAGCTAGGAGTGATGATGACAACTACAATTCT
AGTGACGTTAATCATGCTTCTTATATGGCAGATAAACATCGTAATCGTGATTGCCTTTCT
GGTAGTTTTTCTCGGAGTGGAATTAGTCTTTTTCTCATCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 38-K103549-0022-665-A02C-8409
GenBank Accession LN502948 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K103549-0022-665-A02C-8409
TTTACATTGAATAATCCTGATGAGAAAAAGACTAATTCCACTCCGAGAAAAACTACCAGA
AAGGCAATCACGATTACGATGTTTATCTGCCATATAAGAAGCATGATTAACGTCACTAGA
ATTGTAGTTGTCATCATCACTCCTAGCTCAGCCATCCCTGGGAAATCGAAAAAGAAAATC
AAAACGGGGGAAGCATATCGGATCACCGAAGCTAGACAGGGAAGCATAGAAAACAACTAT
TACCATATGCATTTCCAATCTCATCGATACTTGAGATTGAGCAGACGACGACCAGGCAAA
CGGCAAGCAGGAACCAGTTTAGGACAGGTATATAAATCTGGCCCATAAATTTTCGAGAAG
TGTGAATAATTTTGAGACGAGGGAAACAACCCAGAGCTGTTGANTGTTTA

Last Updated on 06.12.2018 16:11