Sequences from confirmation amplicon


Confirmation sequences for line 666A09 and hit At3g12290 are shown.
Line ID 666A09
Locus At3g12290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3920867
Primers used for PCR 52hw - 8474, show primer details
   
Sequence Name 21-K112877-0022-666-A09C-8409
GenBank Accession LN495737 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K112877-0022-666-A09C-8409
TTCACTTCTTTTGCTCAAGGCTGATGCTACTGTCACAACTGTACATTCTCACACCAAGGA
TCCTGAGGCTATCATACGGGAAGCTGACATTGTTATTGCTGCATGCGGACAAGCCCACAT
GGTGATTTTCATACATTTTCATTGTCATTTAGAAGATAATTTAGTCTACCTCTTGTAGAT
CAATGTGAATAGTTTACAATTCAAATATACTATCGTTTTGACATTTCAGATTAAGGGCAA
CTGGATAAAGCCAGGGGCTGCAGTAATTGATGTTGGAACTAATGCAGTCAGCGACCCGAG
CAAGAAATCAGGATACCGGTTGGTTGGAGATGTTGATTTCGCAGAAGCTTCAAAAGTTGC
AGGTTTCATAACTCCGGTCCCTGGTGGTGTAGGCCCAATGACAGTGGCAATGCTTCTCAG
GAACACCGTAGACGGTGCCAAGCGTGTCTTTGGCGAGTAAAACAATCTACTGTATGTAAT
AAAGAAACCAAGAGTTTCTCCATTCTGTAATTGTGTACTTGGCTTGACGA
   
Sequence Name 58-K112878-0022-666-A09C-52hw
GenBank Accession LN509753 [GenBank]
Sequencing primer gene specific primer, 52hw: 5'-TCGTCAAGCCAAGTACACAATTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K112878-0022-666-A09C-52hw
TAGATTGTTTACTCGCCAAGACACGCTTGGCACCGTCTACGGTGTTCCTGAGAAGCATTG
CCACTGTCATTGGGCCTACACCACCAGGGACCGGAGTTATGAAACCTGCAACTTTTGAAG
CTTCTGCGAAATCAACATCTCCAACCAACCGGTATCCTGATTTCTTGCTCGGGTCGCTGA
CTGCATTAGTTCCAACATCAATTACTGCAGCCCCTGGCTTTATCCAGTTGCCCTTAATCT
GAAATGTCAAAACGATAGTATATTTGAATTGTAAACTATTCACATTGATCTACAAGAGGT
AGACTAAATTATCTTCTAAATGACAATGAAAATGTATGAAAATCACCATGTGGGCTTGTC
CGCATGCAGCAATAACAATGTCAGCTTCCCGTATGATAGCCTCAGGATCCTTGGTGTGAG
AATGTACAGTTGTGACAGTAGCATCAGCCTTGAGCAAAAGAAGTGAAACGGGCAAACCAA
CAATGTTCTAACAGACCCCATTGTCATACTATCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATATCCGCA

Last Updated on 06.12.2018 16:11