Sequences from confirmation amplicon


Confirmation sequences for line 666E12 and hit At3g14390 are shown.
Line ID 666E12
Locus At3g14390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4808936
Primers used for PCR 43sj - 8474, show primer details
   
Sequence Name 38-K116518-0022-666-E12C-43sj
GenBank Accession LN503119 [GenBank]
Sequencing primer gene specific primer, 43sj: 5'-CAAACTTGTGAACAAAAAGCTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K116518-0022-666-E12C-43sj
ATGAGTGTTTATGTGTTTGGACATGGACGACTTTGGCTTGAAACACTCCTCTTATAATCA
GAATCACCTCTTATTTTCTGTCTTGACTTGTGAACGTTTTCTTATTCATATGATCACTTC
ATTGACTTTGAATCAAAGCACATAATGTGAAAATCTAAAACAGCAATATCATTATGGTCC
TGATTCCGAATAATCATACAATTCAATCAAAAAAGCAACAATGATGAGTAAATCTAAGAG
TTCATAGACCTTCAAAGAAATGGCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 12-K116517-0022-666-E12C-8409
GenBank Accession LN491746 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116517-0022-666-E12C-8409
TTGAATATAGCCATTTCTTTGAAGGTCTATGAACTCTTAGATTTACTCATCATTGTTGCT
TTTTTGATTGAATTGTATGATTATTCGGAATCAGGACCATAATGATATTGCTGTTTTAGA
TTTTCACATTATGTGCTTTGATTCAAAGTCAATGAAGTGATCATATGAATAAGAAAACGT
TCACAAGTCAAGACAGAAAATAAGAGGTGATTCTGATTATAAGAGGAGTCTTTCAAGCCA
AAGTCGTCCATTGTCCAAACACATAAACACTCATCTAAGCTTCTTCCCATAGCTATAAGA
AGATTCTTTCAAGCTTTTTGTTCACAAGTTTGAAAGAATCTTCTTNTAGCTATGGGAAGA
AGCTTNGATGAGTGTT

Last Updated on 06.12.2018 16:11