Sequences from confirmation amplicon


Confirmation sequences for line 666G10 and hit At2g22140 are shown.
Line ID 666G10
Locus At2g22140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9418208
Primers used for PCR KR05 - 8474, show primer details
   
Sequence Name 38-K030602-022-666-G10C-8409
GenBank Accession LN502872 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K030602-022-666-G10C-8409
ATTTTTGTGAAAATTTTGTGAAAATCTATAGCCATAACTCATTGGTAATTTTGTGAAAAA
TTGGGGATCTTAGGCAGAAATCATCAGGCTAAACAATATCTCTATGTTTCTTTCGGAAAT
CTCACTAGGCAAACGGATAATAATCTCTGGATTCGGAGTTTGAAGATAGTCCTCGACCTG
AAACTTCGAAGAAGAAATGAATCCGTGCTTGCTGGTTTAAGGGAGCCTCGGTTTGGATTG
GAAGCCGAAACCAGTGAGGCATACTATAAGAATACTAGAATACCTGAGACAAATTTAGGT
ATGCTTACCCCAAAACCAAATTATTGTGCATATATATCAGAGTATACATGATGAACTTCA
TTTGTAGGGTCCACATACATATGAAGTTCATCATGTATACTCTGATATATATGCACAATA
ATTTGGTTTTGGGGTAGCATACCTAAATTGTCTCGGTATTCTAGTATTCTATAGTAT
   
Sequence Name 77-K030602-022-666-G10C-KR05
GenBank Accession LN513478 [GenBank]
Sequencing primer gene specific primer, KR05: 5'-TTGTATGTGGACCCTACAAATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K030602-022-666-G10C-KR05
TATGCACAATAATTTGGTTTTGGGGTAAGCATACCTAAATTTGTCTCAGGTATTCTAGTA
TTCTTATAGTATGCCTCACTGGTTTCGGCTTCCAATCCAAACCGAGGCTCCCTTAAACCA
GCAAGCACGGATTCATTCTTCTTCGAAGTTTCAGGTCGAGGACTATCTTCAAACTCCGAA
TCCAGAGATATTATCCGTTTGCCTAGTGAGATTCCAAAAGAAACATAGAGATATTGTTTA
GCCTGATGATTCTGCCTAAGATCCCCAAATTTTCACAAAATTACCAATGAGTATGGCTAT
AGATTTCACAAAATTTTCACAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAACAAATTATTCTAATTCGGAAAT
CTNTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11