Sequences from confirmation amplicon


Confirmation sequences for line 667E10 and hit At1g05800 are shown.
Line ID 667E10
Locus At1g05800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1742494
Primers used for PCR BD74 - 8474, show primer details
   
Sequence Name 05-K028846-022-667-E10C-8409
GenBank Accession LN488222 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K028846-022-667-E10C-8409
ATCCATGTAGAACAAGTGAACTGATGTTCAGTAAGGGACGACGTCAAGCGTTGCATTTTA
CAAACGCAGCGACCAATGCGGCATATCTACTTTGTTCTATATCCAACCATATGTTGTATT
ATAATATATTTTAGGTCTAGATTGATCCAAGCTAAAATTTTGGATTCTTTTTTAAGATAG
GACAAAATAATTATTGCCCTATCTTTTACGTAGCATAAGAAATGTACATATATAGATGTA
TATGCAAAACCTAATACAAAAAAGTATATTTTCCACATCCAAGATAAATTATTGCGTCTC
ATCTATCACAATGTGAACAAAAATAATTATTTTCTTCTCTTAGTTACTATGAGTTCACAA
TATAATACTTTTCTTTAGGTTAAAGCTCTCAACTGTTGATGAAGTACAAGAACTCAAAAT
TGTGAATGAAAAAAAGGGACATCAT
   
Sequence Name 06-K028846-022-667-E10C-BD74
GenBank Accession LN488674 [GenBank]
Sequencing primer gene specific primer, BD74: 5'-CTGATATTGGATGATGTCCCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K028846-022-667-E10C-BD74
ATTCACAATTTTGAGTTCTTGTACTTCATCAACAGTTGAGAGCTTTAACCTAAAGAAAAG
TATTATATTGTGAACTCATAGTAACTAAGAGAAGAAAATAATTATTTTTGTTCACATTGT
GATAGATGAGACGCAATAATTTATCTTGGATGTGGAAAATATACTTTTTTGTATTAGGTT
TTGCATATACATCTATATATGTACATTTCTTATGCTACGTAAAAGATAGGGCAATAATTA
TTTTGTCCTATCTTAAAAAAGAATCCAAAATTTTAGCTTGGATCAATCTAGACCTAAAAT
ATATTATAATACAACATATGGTTGGATATAGAACAAAGTAGATATGCCGCATTGGTCGCT
GCGTTTGTAAAATGCAACGCTTGACGTCGTCCCTTACTGAACATCAGTTCACTTGTTCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATT

Last Updated on 06.12.2018 16:11