Sequences from confirmation amplicon


Confirmation sequences for line 668G12 and hit At2g28510 are shown.
Line ID 668G12
Locus At2g28510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12199811
Primers used for PCR DC10 - 8474, show primer details
   
Sequence Name 64-K031140-022-668-G12C-DC10
GenBank Accession LN511119 [GenBank]
Sequencing primer gene specific primer, DC10: 5'-CATTATTTCACTCGTCAGCACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K031140-022-668-G12C-DC10
CAACGAGACTTTGGAAACATATTGGTAAGTTCAACAAAAGGAAGCAATAATAACAATAAG
AAAATGGAAGAAGAAATGAAGAAGAAAGTATCAAGAGGAGAATTAGGAGGTGAAGCTCAA
AATTGTCCAAGATGTGAATCTCCAAACACAAAGTTTTGTTACTACAACAACTATAGTCTC
TCACAACCTCGTTACTTCTGCAAATCTTGTCGGAGATATTGGACTAAAGGCGGTACTCTT
CGTAACGTTCCCGTCGGTGGTGGTTGCCGTCGAAACAAACGATCCTCTTCCTCAGCTTTC
TCCAAGAACAACAACAATAAGTCTATTAATTTCCATACTGATCCACTTCAGAACCCTTTA
ATTACGGGAATGCCACCATCATCTTTTGGTTATGATCACTCCATTGATCTCAACCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCTACATTC
   
Sequence Name 29-K031140-022-668-G12C-8409
GenBank Accession LN499169 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K031140-022-668-G12C-8409
ATTTACATTGAATTATCCTGGTTGAGATCAATGGAGTGATCATAACCAAAAGATGATGGT
GGCATTCCCGTAATTAAAGGGTTCTGAAGTGGATCAGTATGGAAATTAATAGACTTATTG
TTGTTGTTCTTGGAGAAAGCTGAGGAAGAGGATCGTTTGTTTCGACGGCAACCACCACCG
ACGGGAACGTTACGAAGAGTACCGCCTTTAGTCCAATATCTCCGACAAGATTTGCAGAAG
TAACGAGGTTGTGAGAGACTATAGTTGTTGTAGTAACAAAACTTTGTGTTTGGAGATTCA
CATCTTGGACAATTTTGAGCTTCACCTCCTAATTCTCCTCTTGATACTTTCTTCTTCATT
TCTTCTTCCATTTTCTTATTGTTATTATTGCTTCCTTTTGTTGAACTTACCAATATAGTT
TCCAAAGTCTCGTTTGAGATTTCCTGCACATTCAAACAAACTAAATTAAATTGTGCTGAC
GATGAAAAATAATG

Last Updated on 06.12.2018 16:11