Sequences from confirmation amplicon


Confirmation sequences for line 669H04 and hit At4g34920 are shown.
Line ID 669H04
Locus At4g34920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16635851
Primers used for PCR 38qz - 8474, show primer details
   
Sequence Name 78-K115869-0022-669-H04C-38qz
GenBank Accession LN513732 [GenBank]
Sequencing primer gene specific primer, 38qz: 5'-TAAAGAGGTCAAACTCCAACCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K115869-0022-669-H04C-38qz
ACGTTTTACCTTAAAAGACAAACACTGCACGATATCTCTCTCAGATTTATAAGTGAACCC
TACAAAGTACAAGTCGTAAACTCAACCACAAGAGAAACCTTATCTTCTTCTTCTTCTTCA
TCATCAATCTCTCAAGACATGCTCTCTTTTTTCTCTAACCAGATCGACCGGCAAAAAGAT
GTGTCTAATGAGGAAAAAACCCTAACCAATCTCGAAAAATCTGACGGTTCACAATTCCCT
GGCGAAACTAATATATATAATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 37-K115867-0022-669-H04C-8409
GenBank Accession LN502738 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K115867-0022-669-H04C-8409
GCCATTTACATTGAATTATCATTATATATATTAGTTTCGCCAGGGAATTGTGATCCGTCA
GATTTTTCGAGATTGGTTAGGGTTTTTTCCTCATTAGACACATCTTTTTGCCGGTCGATC
TGGTTAGAGAAAAAAGAGAGCATGTCTTGAGAGATTGATGATGAAGAAGAAGAAGAAGAT
AAGGTTTCTCTTGTGGTTGAGTTTACGACTTGTACTTTGTAGGGTTCACTTATAAATCTG
AGAGAGATATCGTGCAGTGTTTGTCTTTTAAGTGTAAAACGTTAAATCAAAGACAATTAT
TTGTTGTGGGATTGGTTGGAGTTGACCCTCTTT

Last Updated on 06.12.2018 16:11