Sequences from confirmation amplicon


Confirmation sequences for line 670D06 and hit At2g06530 are shown.
Line ID 670D06
Locus At2g06530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:2589157
Primers used for PCR AS41 - 8474, show primer details
   
Sequence Name 85-K027492-022-670-D06C-8409
GenBank Accession LN514485 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K027492-022-670-D06C-8409
ATCAACCAAGAGGTAAAATACATCAATATCTTAGCTACCATTTACTTATTCCACTTATGG
ATCAATCGAACCAATCTTACCATAGCCGTTAGATATTCACAATAGACCAAATCAGAATCG
GACCTTATCATAGCTTAAACTTCCTCGAACTGAAACCGATACTGCGTGACGTGAGATTTG
GTTTTACTTAACTCGATTGTATCCATCTATAACATTCCTAGTTCTATTTCCCACCTGTAA
GATGCTTAACTCGATTTGACCGATTCATCTATGTTTCAGCTGGTGAATGCTCCATCTGGT
GCGGTCGCAGTACCCGCAGCAAAGAACAAGGTGGTACAAGCTGAGGCGACCGGAGCTGAG
GACAGTGGAGGTATAGATAGTGACCTTCAAGCAAGGTTGGATAACCTTAGAAAAAATGTG
AAGCCTTTCCTGGTTTCTGTATCATAATATTACT
   
Sequence Name 86-K027492-022-670-D06C-AS41
GenBank Accession LN514598 [GenBank]
Sequencing primer gene specific primer, AS41: 5'-AATTGAAAAGTGATCAGCCTCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K027492-022-670-D06C-AS41
GCTTCACATTTTTCAGGGTTATCCACCTTGCTTGAAGGTCACTATCTATACCTCCACTGT
CCTCAGCTCCGGTCGCCTCAGCTTGTACCACCTTGTTCTTTGCTGCGGGTACTGCGACCG
CACCAGATGGAGCATTCACCAGCTGAAACATAGATGAATCGGTCAAATCGAGTTAAGCAT
CTTACAGGTGGGAAATAGAACTAGGAATGTTATAGATGGATACAATCAAGTTAAGTAAAA
CCAAATCTCACGTCACGCAGTATCGGTTTCAGTTCAAGGAAGTTTAAGCTATGATAAGGT
CCATTCTGATTTGGTCTATTGTGAATATCTAACAGCTATGGTAAGATTGGTTCGATTGAT
CCATAAGTGGAATAAGTAAATGGTAGCTAAGATATTGATGTATTTACCTCTTGGTTGATG
TCGATACCGATCTCGTCAAGGACTTGGCTAACAAGATCTTCAGTCTACATGGCATCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTTTTCGACGT

Last Updated on 06.12.2018 16:11