Sequences from confirmation amplicon


Confirmation sequences for line 671B12 and hit At3g17790 are shown.
Line ID 671B12
Locus At3g17790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6089991
Primers used for PCR 10wt - 8474, show primer details
   
Sequence Name 17-K122050-0022-671-B12C-10wt
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 10wt: 5'-AAAAAGAAGAGGTGGATGTCAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K122050-0022-671-B12C-10wt
CGGCTACGGTTGGAAATCCTTCAGCATCTTTAACCGCACCTTTTTTTCTTTTTTACGTAA
TTTTCCTTAAACCACATCTTTAAAATTTTTCGGTTTAAAAAGGATCATCGTCTCCCTCCT
TCTTTCATCATCATCGTTTCCTTCACCATTCACGTTTCTCTTCTTCTCGTCACAAATACC
TTATCTCTCTCTCTCTTTCTCTCTTATATAAAGAGAGAAATATACAAAACCTCCAAGTAC
GTTTCATCGATCCTACCTAATTCATCAAAAAATTGGGGGAGGCTTATTTGATCAATAATT
ATGAATTCGGGTCGTCAATCGTTAATGTCCCCCACGGCAAGTCTGATTTTGCTGTTGTGT
ATCTCCACCACCTTCGTCGGGGTGTCAAACGGTGAGCTTCAAAAATTTATAGAGCCGGCT
AAAAGCGACGGTTCCGTTAGTTTTATAGTCATCGAAAATTGGGGTCGTCGGGGCTCCTTC
AATCAGTCTCTCGTTGCTTATCAGGTTGGGTCCACCGGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACTTGGATCAGCAATGAGTATGAGGGTCAATATGGAAAAAAA
AAAAGAGTATTTACCAATTTTTTTTCAATTCAAAAAAATAAAAAAAAA
   
Sequence Name 01-K122049-0022-671-B12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K122049-0022-671-B12C-8409
TCGGATGAGCATTTCATTGAATATATCCCGGTGGACCAACCTGATAAGCAACGAGAGACT
GATTGAAGGAGCCACGACGACCCCAATCTCCGATGACTATAAAACTAACGGAACCGTCGC
TTTTAGCCGGCTCTATAAATCTCTGAAGCTCACCGTTCGACACCACGACGAAGGTGGTGA
AGATACACAACAGCAAACTCAGACTCGCCGTGGCGGACATTAACGATCGACGACCAGAAT
TCATAATTATTGATCAAATAAGCCTCACACAATTTTTTGATGAATTAGGTAGGATCGATG
AAACGTACTTGGAGGTTTTGTATATTTCTCTCTTTATATAAGAGAGAAAGAGAGAGAGAG
ATAAGGAATTTGTGACGAGAAGAAGAGAAACGTGAATGGTGAAGGAAACGATGATGATGA
AAGAAGGAGGGAGACGATGATCCTTTTTAAACCGAAAAATATTAAAGATGCGGTTAAAGA
AAAATAAAGAAAAAAAGAAAAAAAGGTGCGGTTAAAGATGCTGAAGGATATTCCAACCAG
TAGCCGCATCTTTGTTTTTTTTTTTCTTTTGGGATCTCAAATTCGGACATCCAC

Last Updated on 06.12.2018 16:11