Sequences from confirmation amplicon


Confirmation sequences for line 672C08 and hit At1g32940 are shown.
Line ID 672C08
Locus At1g32940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11937901
Primers used for PCR NC72 - 8474, show primer details
   
Sequence Name 76-K101221-0022-672-C08C-8409
GenBank Accession LN513358 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K101221-0022-672-C08C-8409
TTACATGTAGCTACTTGATAAACTATTCACACCATTATGTATCATGCTGTTGTTAACAAT
GGCTTTTATCTTGTGTTACAGTAAAGTGGATGCTCATGAATCAATGGTGTACAGTTACCG
ACATGGATTTTCAGGCTTTGCTGCCAAGCTTACCGAGTCACAAGCCAAGAAACTTGCTGG
TACTCATAGCTCTCTGTTATCTTGAATTTCACCATGTCTTTGGTTTCTTAGTAATTTGAT
GATAATGATTTAAAATTAGTAATTTACTTTGTTTAGCTGAAATTTTGATTGGTAATGCAC
TTTTGGAAACAGATTCTCCTGAAGTTGTTCATGTCATGGCGGATAGTTTCTATGAACTCG
CTACAACTCGAACTTGGGATTACTTAGGTCTTTCTGTTGCCAATCCAAATAACCTCCTAA
ATGATACAAACATGGGTGACCAAGTTATCATTGGCTTTATTGACACAGGTATAAGTAGTT
CTCATTAGATCGTAGCTTGATCTTAATTCATGTTTCAGATCGTACCTTTATTGAAGCGCC
TCAATGACTTGGCATTTTGTGTGGCTATAGGAGTGTGGCCAGAGTCTGAATCATTTAACG
ACNATGGGGTTGGACCAATACCAAGCCATTGGAAAGGAGGATGTGAATCANGAGAAAAAT
TCATATCAACAAATTGC
   
Sequence Name 76-K101228-0022-672-C08C-NC72
GenBank Accession LN513359 [GenBank]
Sequencing primer gene specific primer, NC72: 5'-GCTTTCTGTTGCAATTTGTTGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K101228-0022-672-C08C-NC72
GCTTGGTATTGGTCCACCCCATTGTCGTTAAATGATTCAGACTCTGGCCACACTCCTATA
GCCACACAAAATGCCAAGTCATTGAGGCGCTTCAATAAAGGTACGATCTGAAACATGAAT
TAAGATCAAGCTACGATCTAATGAGAACTACTTATACCTGTGTCAATAAAGCCAATGATA
ACTTGGTCACCCATGTTTGTATCATTTAGGAGGTTATTTGGATTGGCAACAGAAAGACCT
AAGTAATCCCAAGTTCGAGTTGTAGCGAGTTCATAGAAACTATCCGCCATGACATGAACA
ACTTCAGGAGAATCTGTTTCCAAAAGTGCATTACCAATCAAAATTTCAGCTAAACAAAGT
AAATTACTAATTTTAAATCATTATCATCAAATTACTAAGAAACCAAAGACATGGTGAAAT
TCAAGATAACAGAGAGCTATGAGTACCAGCAAGTTTCTTGGCTTGTGACTCGGTAAGCTT
GGCAGCAAAGCCTGAAAATCCATGTCGGTAACTGTACACCATTGATTCATGAGCATCCAC
TTTACTGTAACACAAGATAAAAGCCATTGTTAACAACAGCATGATACATAATGGTGTTGA
ATAGTTTATCAAGTAGCTACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGT

Last Updated on 06.12.2018 16:11