Sequences from confirmation amplicon


Confirmation sequences for line 672F01 and hit At2g30840 are shown.
Line ID 672F01
Locus At2g30840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13136152
Primers used for PCR CQ69 - 8474, show primer details
   
Sequence Name 24-K107131-0022-672-F01C-CQ69
GenBank Accession LN497015 [GenBank]
Sequencing primer gene specific primer, CQ69: 5'-TTACTCAGATCCCACGTATCTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K107131-0022-672-F01C-CQ69
CGACTAATCTCTTCTTTCCTCTACACGATGGTTATACCAACAATCGATCTTAAAGGAGGC
GTGTTCGACGAGTACACAGTCACGCGAGAGAGCGTCATCGCGATGATCAGAGACGCGGTT
GAGAGATTCGGGTTTTTCCAGGTGATTAACCATGGGATTAGTAATGATGTTATGGAGAAG
ATGAAAGATGGGATTCGTGGGTTTCATGAGCAAGACTCAGACGTTAGGAAAAAGTTTTAT
ACTCGTGACGTCACCAAAACAGTTAAGTATAACAGTAATTTTGATCTTTATAGCTCTCCT
TCAGCGAACTGGAGAGATACCTTGAGTTGCTTTATGGCTCCTGATGTTCCAGAAACAGAG
GACTTACCAGATATTTGTGGGTACGAAATACAAATTTCCTTTATTTCTTAGTCTATAGAA
GAAAGAAAGAAAGAAAGAAGACTTATCTAGTTGTGTGAATGTTTTAGGGAAATCATGTTG
GAATATGCGAAACGAGTGATGAAACTAGGGGAACTAATCTTTGAGCTTTTATCAGAAGCT
TTAGGGCTGAATCCTAACCACTTGAAAGAAATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 08-K107130-0022-672-F01C-8409
GenBank Accession LN489755 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K107130-0022-672-F01C-8409
GCCCTAAAGCTTCTGATAAAAGCTCAAAGATTAGTTCCCCTAGTTTCATCATTCGATTTC
GCATATTCCAACATGATTTCCCTAAAACATTCACACAACTAGATAAGTCTTCTTTCTTTC
TTTCTTTCTTCTATAGACTAAGAAATAAAGGAAATTTGTATTTCGTACCCACAAATATCT
GGTAAGTCCTCTGTTTCTGGAACATCAGGAGCCATAAAGCAACTCAAGGTATCTCTCCAG
TTCGCTGAAGGAGAGCTATAAAGATCAAAATTACTGTTATACTTAACTGTTTTGGTGACG
TCACGAGTATAAAACTTTTTCCTAACGTCTGAGTCTTGCTCATGAAACCCACGAATCCCA
TCTTTCATCTTCTCCATAACATCATTACTAATCCCATGGTTAATCACCTGGAAAAACCCG
AATCTCTCAACCGCGTCTCTGATCATCGCGATGACGCTCTCTCGCGTGACTGTGTACTCG
TCGAACACGCCTCCTTTAAGATCGATTGTTGGTATAACCATCGTTGTAGAGGAAAGAAGT
AGATTTGAGTCGGTTAAATTAAGATGTGGATGATGGAAGATACGT

Last Updated on 06.12.2018 16:11