Sequences from confirmation amplicon


Confirmation sequences for line 672G02 and hit At3g60390 are shown.
Line ID 672G02
Locus At3g60390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22321077
Primers used for PCR 65rn - 8474, show primer details
   
Sequence Name 66-K116102-0022-672-G02C-65rn
GenBank Accession LN511686 [GenBank]
Sequencing primer gene specific primer, 65rn: 5'-ATTGAATCTGAGGACGAGACAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K116102-0022-672-G02C-65rn
TTTTTATTCTATTTAAGGGGTTACTTTCGTAAATTTTATATAGTCCAACAAATAAAACAT
TAGTAGTTATTGCTATGATGGTGATATGTGTAATGGTCCATTTTATGACCCATAAGATTA
TCCTAAAGGATTTTAATGGGTCTCTCATGTCTTAAATTAGGAGGTTTGGTTTAAGCTATT
CAGTTTTCCCGGTTTAAGAATTAAAACCGCCAATGTTGTTTTTGAGTCAAATTTTGGTTA
AAGTGTTTTCAGTTTTCCGGTTCAAGAACAATAACCGCCAGTTTCGAATACAAGAAACAA
ATGTACTTCGGGTTTTCTCTTTCCCGGATAGTTAAACCGGATATCGGATTTTAATGTTTT
GGACTGTTTGTGTGTGTGTTTATAGGACGAAGCTGAAGCAAACGGATGTCTACATGGCTT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCA
   
Sequence Name 48-K116101-0022-672-G02C-8409
GenBank Accession LN506606 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K116101-0022-672-G02C-8409
TGAAGCCATGTAGACATCCGTTTGCTTCAGCTTCGTCCTATAAACACACACACAAACAGT
CCAAAACATTAAAATCCGATATCCGGTTTAACTATCCGGGAAAGAGAAAACCCGAAGTAC
ATTTGTTTCTTGTATTCGAAACTGGCGGTTATTGTTCTTGAACCGGAAAACTGAAAACAC
TTTAACCAAAATTTGACTCAAAAACAACATTGGCGGTTTTAATTCTTAAACCGGGAAAAC
TGAATAGCTTAAACCAAACCTCCTAATTTAAGACATGAGAGACCCATTAAAATCCTTTAG
GATAATCTTATGGGTCATAAAATGGACCATTACACATATCACCATCATAGCAATAACTAC
TAATGTTTTATTTGTTGGACTATATAAAATTTACGAAAGTAACCCCTTAAAATAGAATAA
AAATTTCTACCTTGCCCTTCGGTTTTGGAACCACACTTCAACTTGTCTCGTCTCAGAATT
CAATA

Last Updated on 06.12.2018 16:11