Sequences from confirmation amplicon


Confirmation sequences for line 672G10 and hit At2g31790 are shown.
Line ID 672G10
Locus At2g31790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13518738
Primers used for PCR NS31 - 8474, show primer details
   
Sequence Name 93-K102372-0022-672-G10C-8409
GenBank Accession LN515245 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K102372-0022-672-G10C-8409
ATTTACATTGAATATATCATTGCAATATACATTAAAAGAGACAGAACACAAAACGTTTCG
TAATTGACAAAAATAGAAATTCTATGTCACTCGGAAGTCCAGGATATAGATTTTTGACTA
AAATTGTTCTCTCATCAAATTCCAAAGTAAAATTACCTCTAAAATAACTAAGGAGATATG
TCATATTGGAAATTCATGTATTGTACCTAGAGATGAAATCGGTCAGGCTTCGAGAAGTAG
AGTTGTGGAAACGGTCAAGATCTACGAACTTGGCGTGAGGGTGTTCATGTGGAAAGAAAC
CGTCGTGGATGGTGTGGACGGTGATGGAGTAGTCGTCGGAGGTGTAAGGTTCACGGTGGT
CTTTGGAGGCGATGATGAGTGTGCTGGTGATGCCCTTTTTGGATAAGCGTTTAGCGAGTT
GGATCATTGGGTTAATGTGGCCTTGTAATGGATATGGAAAAAACAGTACGTGACCCTTCT
TTGCTTCACTCATTTTTTTGGTAAAATTGTTCAAACNAAAGGAA
   
Sequence Name 93-K102373-0022-672-G10C-NS31
GenBank Accession LN515246 [GenBank]
Sequencing primer gene specific primer, NS31: 5'-GTTCCTTTGGTTCGAACAATTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K102373-0022-672-G10C-NS31
GTACTGTTTTTTCCATATCCATTACAAGGCCACATTAACCCAACGATCCAACTCGCTAAA
CGCTTATCCAAAAAGGGCATCACCCGCACACTCATCATCGCCTCCAAAGACCACCGTGAA
CCTTACACCTCCGACGACTACTCCATCACCGTCCACACCATCCACGACGGTTTCTTTCCA
CATGAACACCCTCACGCCAAGTTCGTAGATCTTGACCGTTTCCACAACTCTACTTCTCGA
AGCCTGACCGATTTCATCTCTAGGTACAATACATGAATTTCCAATATGACATATCTCCTT
AGTTATTTTAGAGGTAATTTTACTTTGGAATTTGATGAGAGAACAATTTTAGTCAAAAAT
CTATATCCTGGACTTCCGAGTGACATAGAATTTCTATTTTTGTCAATTACGAAACGTTTT
GTGTTCTGTCTCTTTTAATGTATATTGCAATGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATACATACAAAGAGACAG

Last Updated on 06.12.2018 16:11