Sequences from confirmation amplicon


Confirmation sequences for line 673C04 and hit At3g22942 are shown.
Line ID 673C04
Locus At3g22942 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8135076
Primers used for PCR IW56 - 8474, show primer details
   
Sequence Name 34-K033213-022-673-C04C-8409
GenBank Accession LN501314 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K033213-022-673-C04C-8409
CATTTACGAAGCATATTGTTCGCAAAGGATCTACTCTCTCACTCAAGTAACATTTAGCAT
CACAAAAATCTCTTTAAAAGGAGACAGTTCTACACTACATTTCTTAAAGTCAGATCGATT
CCATTAATCTAAGATCCAATCTTGTTAAGCATATAAAAAGGAACTATATCAAGAGACAAG
TACTATTAACTCACTAAAAGCAACCCCACAATACCAACTGCTACAACAAAACCTGTTTTT
TTTTTTTCCCTCATTGAATACCAACAACAACAAAAACCCCTAAATCAAATTGATTCCCAA
AACTAAAAAAGTTTCAAACTTTGAAAGTCTCTACCGTACCCCACTCAAATTTATACTATT
CCATCCAAAAAGAGCCAAACCACAAAAACAACAAATTGATCCAATCTGTAATTATATGAT
ATAATCAGCCAAAAGGCCCAAGCACGTCAAAACAAGAAAGGGTTTAAAAA
   
Sequence Name 42-K033213-022-673-C04C-IW56
GenBank Accession LN504365 [GenBank]
Sequencing primer gene specific primer, IW56: 5'-TCTTAAACCCTTTCTTGTTTCGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K033213-022-673-C04C-IW56
TAATTACGATTGGATCAATCTGTTGTTTTTGTGCTTCGGCTCTTTTTGGATGGAATAGTA
TAAATCTGAGTGGAGTACGGTAGAGACTTTCAAAGTTTGAAACTTTTTTAGTTTTGGGAA
TCAATTTGATTTAGGGGTTTTTGTTGTTGTTGGTATTCAATGAGTGAAAAAAAAAAAACA
GGTTTTGTTGTACCATTTGGTATTGGGGGGTTGCTTTTAGTGAGTTAAAAGTACTTGTCC
CTTGATATAGTTCCTTTTTATATGCTTAACAAGATTGGATCTTAAATTAAGGGAATCAAT
CTGACTTTAAAAAATGTAGTGTAAAACTGTCCCCTTTTAAAAAAATTTTTGTGATGCTAA
ATGTTACTTGAGTGAAAAAGTAAATCCTTTGCAAACAATATGCTTCTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCACCAATGAGTATGATGGTCAATATGGAAAAAAAAAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCCCACCGTTATTATAAAA
TGAAAGTACTTTTTGATAAAACGACAAATTACAATCCGTCGTATTTATAGGCAAAAGCAA
TAAACAAATTATTCTAATTCGAAAATCTTTATTTCAACGTGTCTACATTC

Last Updated on 06.12.2018 16:11