Sequences from confirmation amplicon


Confirmation sequences for line 675D01 and hit At1g26850 are shown.
Line ID 675D01
Locus At1g26850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9301345
Primers used for PCR 18os - 8474, show primer details
   
Sequence Name 62-K114954-0022-675-D01C-18os
GenBank Accession LN510821 [GenBank]
Sequencing primer gene specific primer, 18os: 5'-AGAAGAAACAGAGTTGGCTAGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K114954-0022-675-D01C-18os
CACAAAGTTCCAGCTTTTGGATTGGGAAACAAAAATAAACTATAACATCAATAAATAGAT
ACTTCAAAAAGTGTTTAAAGACAAGATTTGCAGCTCTCTCACATTCCCTAAGGACGAAAG
CTCTATCATTTGATCAAAATGTCAGGTCACACCGGAAATAAAGTAACTTCAGTGAGTCGA
GGTGGAGTTGGTGACCCAATACTGCTTCACGGCAATCAAAACCTTCTCAGGAACTAAAGG
ACCATCCTCATGATCGACAAGCTTCGCATCCCATCTCATTCCAGCGATGATTCTCTTCAC
CTTGATCAATGTATCCACATCATCCCGGATTATAACCGCTCCTTCTGGTCGGAGAATTCT
ATCCATCTCTAGAAGAATATCATCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCA
   
Sequence Name 28-K114953-0022-675-D01C-8409
GenBank Accession LN498970 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114953-0022-675-D01C-8409
AGATGGATAGAATTCTCCGACCGAAGGAGCGGTTATAATCCGGGATGATGTGGATACATT
GATCAAGGTGAAGAGAATCATCGCTGGAATGAGATGGGATGCGAAGCTTGTCGATCATGA
GGATGGTCCTTTAGTTCCTGAGAAGGTTTTGATTGCCGTGAAGCAGTATTGGGTCACCAA
CTCCACCTCGACTCACTGAAGTTACTTTATTTCCGGTGTGACCTGACATTTTGATCAAAT
GATAGAGCTTTCGTCCTTAGGGAATGTGAGAGAGCTGCAAATCTTGTCTTTAAACACTTT
TTGAAGTATCTATTTATTGATGTTATAGTTTATTTTTGTTTCCCAATCCAAAAGCTTGGA
ACTTTGTGGTGTACCATTGAATTCTCAACTTAATAGTTTTTGCACTAGCCAACTTGTTTT
CTTC

Last Updated on 06.12.2018 16:11