Sequences from confirmation amplicon


Confirmation sequences for line 676E10 and hit At1g22020 are shown.
Line ID 676E10
Locus At1g22020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7755280
Primers used for PCR 48pv - 8474, show primer details
   
Sequence Name 26-K115400-0022-676-E10C-48pv
GenBank Accession LN498061 [GenBank]
Sequencing primer gene specific primer, 48pv: 5'-GACGATGAGGTTGTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115400-0022-676-E10C-48pv
GACATTTTTCTCTTGGGTCATCCTATGAAGTTGAACGAGGTAGAGGTGGGAATTCGTATT
CATTGGCATCATCAAGTCCTTGTAAGAGATTTGTAGTTGATTCAGGGATTGAAAGTAGGC
GAGCTGTTGTTAGAGCTTGGGGTAATCAGTCGATTGAAGAAGCTGATCCTGAGATTCATG
AGTTTATGGAAAAGGAGAAGCAGAGACAATTTAGAGGTATTGAGCTTATAGCTTCTGAGA
ATTTCGTGTGTAGAGCTGTGATGGAAGCTTTAGGAAGTCATTTGACTAATAAGTACTCTG
AAGGAATGCCTGGAGCTAGATACTATACGGGGAATCAGTATATTGATCAGATTGAGATTC
TTTGCCAAGAGCGTGCGCTTGCAGCTTTTGGACTAAATCATGAGAAATGGGGAGTTAATG
TGCAGCCATTGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 02-K115399-0022-676-E10C-8409
GenBank Accession LN487163 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115399-0022-676-E10C-8409
ACTCCCATTTCTCAGATTTAGTCCAAAGCTGCAAGCGCACGCTCTTGGCAAAGAATCTCA
ATCTGATCAATATACTGATTCCCCGTATAGTATCTAGCTCCAGGCATTCCTTCAGAGTAC
TTATTAGTCAAATGACTTCCTAAAGCTTCCATCACAGCTCTACACACGAAATTCTCAGAA
GCTATAAGCTCAATACCTCTAAATTGTCTCTGCTTCTCCTTTTCCATAAACTCATGAATC
TCAGGATCAGCTTCTTCAATCGACTGATTACCCCAAGCTCTAACAACAGCTCGCCTACTT
TCAATCCCTGAATCAACTACAAATCTCTTACAAGGACTTGATGATGCCAATGAATACGAA
TTCCCACCTCTACCTCGTTTCAACTTCATAGGATGACCCAAGAGAATAAATTGCTCCTCT
TCTCTTTGATCATCATCATCATTATCACCAACAACCTCAT

Last Updated on 06.12.2018 16:11