Sequences from confirmation amplicon


Confirmation sequences for line 676F10 and hit At5g41100 are shown.
Line ID 676F10
Locus At5g41100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16447921
Primers used for PCR 13jk - 8474, show primer details
   
Sequence Name 49-K113214-0022-676-F10C-8409
GenBank Accession LN506903 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K113214-0022-676-F10C-8409
TTGAATATATCCTGAGTCTTTGAATATATGAGGGTTATGATAGATTACTCGAAGTAGCTG
CTGCTATGGCTAATAGCGCATATGGTAAATAAGCATTCAACCTTTATGAATCTCCACTTC
TATAGCTTCATGTATGAATTTGGAAGGTTGATGTTGTCAAACATTGTGACTGTAAAGTGT
ACGGTTGCTTCATATAAGATGGGGCTTGCTATACCCGAGCTTTATTGGATTGAATTAGCT
TTCATTCATATTGTTTCTAGCGGAGGTCTTCGATAATATTATCATTTTGCGAAATCTTCA
TTTTTAGTGTCTCTAGAATTTCCCATTAAGTACTTTCTTAACCTTGTGAGAAACCTTTGT
TGATTCATATAAGAACATCAGTATATTGTCAGACCTCATCATTTTTTTTTTTTCTTTGGT
CCGGAAAAATCAAAGTTCTCTAAATCATTGGGGAAAATGGGCTCATGCTTGAACCAAATC
GCCCCTCAAAATGATCAAAAAAGGGGTAAAATTTTGGGTATTTACCTTTCAGGGACTTTT
GTTTGCCCGGTTTCCATGCTGAAACATTGTTTCTTCTGCAGGT
   
Sequence Name 49-K113215-0022-676-F10C-13jk
GenBank Accession LN506904 [GenBank]
Sequencing primer gene specific primer, 13jk: 5'-ATTAGAAGAATTCCACCTGCAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K113215-0022-676-F10C-13jk
CGTCCTGATAGCTAAATACCCAAAATCTTACCACTTTCTTGATCATTATGAGGCGCGATT
TGCTCCAAGCATGAGCCCATTTCCCCCAATGATTCAGAGAACTCTGATTATACCGAACAA
AAGAAAAAAAAAAAATGATGAGGTCGGACAATATACGGATGTTCTTATTTGAATCAACAA
GGGTTTCTCACAGGGTTAAAAAAGTACTTAAGGGGAATTTCTAGAGACCCTAAAAATGAA
GATTTCGCAAAAGGATAATTTTATCGAAAACCTCCGCTAGAAACAATATGAATGAAAGCT
AATTCATTCCAATAAAGCTGGGGTATAGCAGGCCCCTTCTTATATGAAGCACCCGTACAC
TTTACAGTCCCAATGTTGGACAACTTCAACCTTCCAAATTCATACTTGAAGCTTTGGAAG
TGGAGATTCATAAGGGTTGAAGGCTTATTTACCATATGCGCTTTTAGCCATAGCAGCAGC
TACTTCGAGTAATCTTTCAAACCCCTCAAATTTCCAAAGACTCAGGATATTTTCATTTGT
AAAGGGCTTCATGTCCGGGAAATCTACTGGGATCACCAATGAGTATGAGGGTCAATATGG
AGAAAAAGAAAGAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATTTCC

Last Updated on 06.12.2018 16:11