Sequences from confirmation amplicon


Confirmation sequences for line 676G08 and hit At1g09665 are shown.
Line ID 676G08
Locus At1g09665 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3133555
Primers used for PCR NG89 - 8474, show primer details
   
Sequence Name 95-K102372-0022-676-G08C-OriL
GenBank Accession LN515346 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K102372-0022-676-G08C-OriL
ATTCTTTACCTTAGCTTACTCATCATCCCTAATCTAATATTGCGACATTTTTCAGTTGTC
GGTTTCCCTTTTGGTTGTCGTTAACAGAACAAACAAATGGGTCCAGATGTACATAAATGG
CTAAAGATGTAAGAGTTGAAAACGATGACTTGGCTTATTTCTTTGTAGTCGTTAACAAAA
TATGATTTCTTCACTGATTCAATAACTTTCACCTTTGTAATAAATAACACTTCATTCTGT
GAAACTATTGAGGCTGAACTACTTTTCAAATACTTTGCAGCTCCGAGGCGGATTTCATAA
AGGAAATTGTTGGAGAGGTCAAAAAGAGTAGTCGTCGCCGTTGAGGAAATGGGAGATTGA
TCCTCTTACATAAACAGACTAAAATCGTTAATGTAGCCAAGCTGACCAAATCCACTAAAA
GAAA
   
Sequence Name 95-K102373-0022-676-G08C-NG89
GenBank Accession LN515347 [GenBank]
Sequencing primer gene specific primer, NG89: 5'-GTTTCTTTAGCTGGATTTGGTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K102373-0022-676-G08C-NG89
GAGGATCAATCTCCCATTTCCTCAACGGCGACGACTACTCTTTTTGACCTCTCCAACAAT
TTCCTTTATGAAATCCGCCTCGGAGCTGCAAAGTATTTGAAAAGTAGTTCAGCCTCAATA
GTTTCACAGAATGAAGTGTTATTTATTACAAAGGTGAAAGTTATTGAATCAGTGAAGAAA
TCATATTTTGTTAACGACTACAAAGAAATAAGCCAAGTCATCGTTTTCAACTCTTACATC
TTTAGCCATTTATGTACATCTGGACCCATTTGTTTGTTCTGTTAACGACAACCAAAAGGG
AAACCGACAACTGAAAAATGTCGCAATATTAGATTAGGGATGATGAGTAAGCTAAGGTAA
ATGAATATGATGAGTAAATATGGAGATCTACATGGATCAGCAATGAGTATGATGGTCAAT
AT
Primers used for PCR NG88 - 8474, show primer details
   
Sequence Name 94-K102372-0022-676-G08C-8409
GenBank Accession LN515298 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K102372-0022-676-G08C-8409
ATTGTTGTCTTTTCTTTTTCGCCTAACGTTAAGCCCACCTTGTCAGAAACAGACTCTAAA
GCTTCCTTCCATTTCTTGATTTGTTCTCCACTCGAAGTCTTAGCCAACCTCCAGAAGTTG
CGACCAAACTCAGCCTTTTGATATCTAACAGACTCTGCCTTCACTTTAAAGAAGATAGGA
ACCACTCGTAGCTTGCATTTATCGGCTCTTTTCTTGATCCTTGCAAGCTCGTCCAGGCAC
CAGCTTGACTCTGCGTATCTTTTGGAAAATATGGTGAGAGCCATCCTCGATTGCTTGATC
CTTGCAAAGAGATGTTTGAGGTCTTTCCCTTTTTGTTCGTCTTTGTCTATGAAGAAGTTG
ATTCCATGTCTTTTAAAAGCGTCAACGAGGTGGCTCACAAAGCTGCAGCGTAGCTCCTCT
CCCCGGAAATTGATGAACACTTGGATCTTCCTATTACTACTACTCCTTTTCATGTTGTTT
AATTTCCTTTAGTTGTGAGCGTGAGCAATGAAGAGAAGTTGGTTAGACTTTGTTCCACTC
TTATACAATTTTGTTTGGACAGAAGACAAGTTTTGTAATGTAATATGGAACAATGGTTAC
TAAGATGTTTAGCAAGAGCTTAATTTATAGTATATGATATTGGGATTTGGGAGGGTTTAG
GTACATTGTTAATGTTAGGTTTATCGATATTACTGGAGGGAAGGCGTCGTTAGGAACTTT
GTCATTAACTTTCTTGAGTTAAC
   
Sequence Name 94-K102373-0022-676-G08C-NG88
GenBank Accession LN515299 [GenBank]
Sequencing primer gene specific primer, NG88: 5'-GCATTTACGCATGTTAACTCAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K102373-0022-676-G08C-NG88
TACGACCCTTCCTCCAGTATATCGATAAACCTAACATTAACAATGTACCTAAACCCTCCC
AAATCCCAATATCATATACTATAAATTAAGCTCTTGCTAAACATCTTAGTAACCATTGTT
CCATATTACATTACAAAACTTGTCTTCTGTCCAAACAAAATTGTATAAGAGTGGAACAAA
GTCTAACCAACTTCTCTTCATTGCTCACGCTCACAACTAAAGGAAATTAAACAACATGAA
AAGGAGTAGTAGTAATAGGAAGATCCAAGTGTTCATCAATTTCCGGGGAGAGGAGCTACG
CTGCAGCTTTGTGAGCCACCTCGTTGACGCTTTTAAAAGACATGGAATCAACTTCTTCAT
AGACAAAGACGAACAAAAAGGGAAAGACCTCAAACATCTCTTTGCAAGGATCAAGCAATC
GAGGATGGCTCTCACCATATTTTCCAAAAGATACGCAGAGTCAAGCTGGTGCCTGGACGA
GCTTGCAAGGATCAAGAAAAGAGCCGATAAATGCAAGCTACGAGTGGTTCCTATCTTCTT
TAAAGTGAAGGCAGAGTCTGTTAGATATCAAAAGGCTGAGTTTGGTCGCAACTTCTGGAG
GTTGGCTAAGACTTCGAGTGGAGAACAAATCAAGAAATGGAAGGAAGCTTTAGAGTCTGT
TTCTGACAAGGTGGGCTTAACGTTAGGCGAAAAAGAAAAAGAACAAACAAATTACCAATG
GATCAGCAATGAGTATGAGGGTCAAT

Last Updated on 06.12.2018 16:11