Sequences from confirmation amplicon


Confirmation sequences for line 677C12 and hit At1g09610 are shown.
Line ID 677C12
Locus At1g09610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3112380
Primers used for PCR CQ72 - 8474, show primer details
   
Sequence Name 25-K107131-0022-677-C12C-CQ72
GenBank Accession LN497468 [GenBank]
Sequencing primer gene specific primer, CQ72: 5'-AGATTTCTTTACTGGGCTGAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K107131-0022-677-C12C-CQ72
CACTTGGTTTACGTAATAAGAAAGTCTAGTTTCTTGCTATAGCTTTATGTCTAGGCTCTC
CAATACTGGTTGTGGACAAGTAGGTGAACAACAAGGACGATAAAGAGTTCCATATTTGGA
GAAACAGAGGAGATCTCTAGGTAACATGTGTCACTATGGACAAAAGGGTCTATTTGATTC
TGATTCTGATCCATCTCTATAGCTAGGGATAATAAAATGCCTTAGTCTCCCTTCCTGTTT
CTTCATGTACCCTTCACACAAGAAAGCCTTAGAAAACTTGTCTTCTATTTCCCTGTTAAC
ATCATGCACAAACACATCAGTCTCTCCTCCCTGTTTCCTGTTCCTTGCCATCATTCCCGC
AGTGTAAATTGCTGTCATTCTCCCAGGAGCCTCATCGTAGTAGCCAGTCGGTGCATCAAC
CATGATTAGCAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 09-K107130-0022-677-C12C-8409
GenBank Accession LN490203 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K107130-0022-677-C12C-8409
TGCCCGACTGGCTACACGATGAGGCTCCTGGGAGAATGACAGCAATTTACACTGCGGGAA
TGATGGCAAGGAACAGGAAACAGGGAGGAGAGACTGATGTGTTTGTGCATGATGTTAACA
GGGAAATAGAAGACAAGTTTTCTAAGGCTTTCTTGTGTGAAGGGTACATGAAGAAACAGG
AAGGGAGACTAAGGCATTTTATTATCCCTAGCTATAGAGATGGATCAGAATCAGAATCAA
ATAGACCCTTTTGTCCATAGTGACACATGTTACCTAGAGATCTCCTCTGTTTCTCCAAAT
ATGGAACTCTTTATCGTCCTTGTTGTTCACCTACTTGTCCACAACCAGTATTGGAGAGCC
TAGACATAAAGCTATAGCAAGAAACTAGACTTTCTTATTACGTTAAACCAAGTGTTTTGT
AAAAAAGACCATTTAACTTTGTTTCAGCCCAGTAAAGGAAAT

Last Updated on 06.12.2018 16:11