Sequences from confirmation amplicon


Confirmation sequences for line 678H09 and hit At5g13260 are shown.
Line ID 678H09
Locus At5g13260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4245723
Primers used for PCR 11bn - 8474, show primer details
   
Sequence Name 51-K107839-0022-678-H09C-11bn
GenBank Accession LN507524 [GenBank]
Sequencing primer gene specific primer, 11bn: 5'-CTAGAACTTTCATGCCATCGTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K107839-0022-678-H09C-11bn
GAATATGTTCGGATATAGCTACGTCCAAGTACGAATACTGGTCATCTCTGGCTCCTCTTC
CTTTTGAAATTGTGCTGTCAGCTGGACAGAAGGCTAAAGAAGAAAGTTGGGAAAAAGGCA
AGCAATCACTCAATCATATTTCTAAGATTCTGTTGTGATAACATTCTATAACTCCGAGTA
TCACTTCTCGTTACTGTATATTTATCTGTTGGCTATGAAAACATCTGACCAGAATCTGAG
GAGAACGAGAAGAGGAGCCAGCTCGTTCAAGATATAAATGATCTAACAGGAGAAGGAAAT
ATTGAAAGTATGCTCTCTGTGGAAATGGGTCTAAAGGAGCTAACTTCTTTAAAGGTTTAT
TCTCATCCTCTCTGGTTCTGGTTCATGCGTCCCTCTTTCATTTTCTCATGGACTATACTT
GTTCTTTTGAAGGTTGAAGTTGCCATTACTATTACATTAGCCCAGCTAAGGTTGGCCAAT
ACTACTCGACTGTCTGATATCGGTGAGCTTCTTCTTCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATT
A
   
Sequence Name 83-K107840-0022-678-H09C-8409
GenBank Accession LN514321 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K107840-0022-678-H09C-8409
GCATTTACATTGAATATATCCTGAAGAAGAAGCTCACCGATATCAGACAGTCGAGTAGTA
TTGGCCAACCTTAGCTGGGCTAATGTAATAGTAATGGCAACTTCAACCTTCAAAAGAACA
AGTATAGTCCATGAGAAAATGAAAGAGGGACGCATGAACCAGAACCAGAGAGGATGAGAA
TAAACCTTTAAAGAAGTTAGCTCCTTTAGACCCATTTCCACAGAGAGCATACTTTCAATA
TTTCCTTCTCCTGTTAGATCATTTATATCTTGAACGAGCTGGCTCCTCTTCTCGTTCTCC
TCAGATTCTGGTCAGATGTTTTCATAGCCAACAGATAAATATACAGTAACGAGAAGTGAT
ACTCGGAGTTATAGAATGTTATCACAACAGAATCTTAGAAATATGATTGAGTGATTGCTT
GCCTTTTTCCCAACTTTCTTCTTTAGCCTTCTGTCCAGCTGACAGCACAATTTCAAAAGG
AAGAGGAGCCAGAGATGACCAGTATTCGTACTTGGACGTAGCTATATCCGAACATATTCC
TGAAATGCAAATATGCACATATGTCAAGAAGCAAAGATACACGATGGCATGAAAGTTCTA

Last Updated on 06.12.2018 16:11