Sequences from confirmation amplicon


Confirmation sequences for line 679H10 and hit At1g05500 are shown.
Line ID 679H10
Locus At1g05500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1627430
Primers used for PCR KU79 - 8474, show primer details
   
Sequence Name 12-K103309x-0022-679-H10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103309x-0022-679-H10C-8409
ATTTACATTGAATATATCCTGAAGTTGAACTGTTGACGTTCTTCAGTGGAGGATATGCTC
AACTTTTTTTTCTTTTCGTTTTGACTTTCTTACTATTGTGTTATAGCACTTTCGAATAGT
CAATGCAAATGATGGTGCTTGTCTTTCTGGTTTTGGACTTTTATTTTAGATTTTTTTGTG
TTTCTATTCCCAGCGATCTGGAGCTAAAGCCTGTTGGAATGTTAGAGGTGAAGCTTGTGC
AAGCAAAGAACTTGACCAACAAAGATCTAGTTGGAAAATCAGACCCCTTTGCTAAAATGT
TTATACGCCCTTTGCGTGAAAAGACTAAAAGAAGCAAGACAATTGTAAGTAGTCCATCTA
ATCCTCTTTTTGTGCTAAACCTACCAGAATGATCTGATTTATATTTATTCTCATCAGAAC
AATGACCTGAATCCCATCTGGAATGAGCATTTTGAATTTGTTGTGGAAGACGCGTCAACT
CAACATTTAGTGGTCAGAATATATGATGACGAGGGTGTACAAGCATCTGAGCTTATTGGT
TGTGCCCAAATCCGGCTTTGTGAACTTGAACCTGGTAAAGTGAAAGACGTCTGGTTGAAG
CTGGTCAAAGATTTGGAGATCCAGAGAGATACTAAGAAACCGTGG
   
Sequence Name 04-K103310x-0022-679-H10C-KU79
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, KU79: 5'-CCACGGTTCTTAGTATCTCTCTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K103310x-0022-679-H10C-KU79
CTTCACCAGACGTCTTTCACTTTACCAGGTTCAAGTTCACAAAGCCGGATTTGGGCACAA
CCAATAAGCTCAGATGCTTGTACACCCTCGTCATCATATATTCTGACCACTAAATGTTGA
GTTGACGCGTCTTCCACAACAAATTCAAAATGCTCATTCCAGATGGGATTCAGGTCATTG
TTCTGATGAGAATAAATATAAATCAGATCATTCTGGTAGGTTTAGCACAAAAAGAGGATT
AGATGGACTACTTACAATTGTCTTGCTTCTTTTAGTCTTTTCACGCAAAGGGCGTATAAA
CATTTTAGCAAAGGGGTCTGATTTTCCAACTAGATCTTTGTTGGTCAAGTTCTTTGCTTG
CACAAGCTTCACCTCTAACATTCCAACAGGCTTTAGCTCCAGATCGCTGGGAATAGAAAC
ACAAAAAAATCTAAAATAAAAGTCCAAAACCAGAAAGACAAGCACCATCATTTGCATTGA
CTATTCGAAAGTGCTATAACACAATAGTAAGAAAGTCAAAACGAAAAGAAAAAAAAGTTG
AGCATATCCTCCACTGAAGAACGTCAACAGTTCAACTTCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTAATTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11