Sequences from confirmation amplicon


Confirmation sequences for line 683B06 and hit At1g30910 are shown.
Line ID 683B06
Locus At1g30910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11001521
Primers used for PCR 21cv - 8474, show primer details
   
Sequence Name 30-K109234-0022-683-B06C-21cv
GenBank Accession LN499756 [GenBank]
Sequencing primer gene specific primer, 21cv: 5'-GCTGTTTCTTCATCTGGGTTTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K109234-0022-683-B06C-21cv
GGGTTCATCATCATTCAGTGAGACTCCTTGTTGGTGGGTCTACGTTGTCTTTAGTGTGAC
TCTGAGTCTCTGAAAGTTATTAAGTGGAAAGATTGATTTTTGTGCTGTCCTCAATGATTC
TCTAAAAGTGTATAAGACTTGGAATGTGAATGATTAAGTGTGAAGTAATCCAAAACAGTT
TAATGAAGTTGTCATTGTGAGAGCTGATGTGATTATTTTGGTGTGATTTGTTTGGGGAAA
ATTAGAAAAGCTAAGTTTTTTTTGGGGTTTGGTTGCTTAGGATTTCGATGGGATCGAAAC
TGGTTGATTGTGAATTCTAAAGGAAGAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 14-K109233-0022-683-B06C-8409
GenBank Accession LN492547 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K109233-0022-683-B06C-8409
CATTTACAATTGAATATATCCTCTTCCTTTAGAATTCACAATCAACCAGTTTCGATCCCA
TCGAAATCCTAAGCAACCAAACCCCAAAAAAAACTTAGCTTTTCTAATTTTCCCCAAACA
AATCACACCAAAATAATCACATCAGCTCTCACAATGACAACTTCATTAAACTGTTTTGGA
TTACTTCACACTTAATCATTCACATTCCAAGTCTTATACACTTTTAGAGAATCATTGAGG
ACAGCACAAAAATCAATCTTTCCACTTAATAACTTTCAGAGACTCAGAGTCACACTAAAG
ACAACTTAGACCCACCAACAAGGAGTCTCACTGAATGATGATGAACCCTTAAACAACGGG
CTAATCGAAAATATCGTAAGAAGACTTAAACCCAGATGAAGAAACAG

Last Updated on 06.12.2018 16:11