Sequences from confirmation amplicon


Confirmation sequences for line 683E11 and hit At3g20830 are shown.
Line ID 683E11
Locus At3g20830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7285062
Primers used for PCR AB64 - 8474, show primer details
   
Sequence Name 42-K105566-0022-683-E11C-AB64
GenBank Accession LN504464 [GenBank]
Sequencing primer gene specific primer, AB64: 5'-TCCAAATCCAATACCCACTAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K105566-0022-683-E11C-AB64
TCAAATAAACTAATGACTCCTTATAATTATACCTTTTAAAAAATCATAGTTTTATCTAAA
TTTCGCTATATTTTACAAATATTTTCTTCGGATTGTGCAAGCGAAGTCTTCAACACCAAA
AGATTATAATTTTCATAATTCTCACACGTTAGGGGGTTATGGATATACATTTTCTCTACA
ACTTTGTACGTGTTAGATTCCCCACACGTGGATTAGAATTCAATAAACGGATCGTTTTTA
CGACACACGTGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCG
   
Sequence Name 74-K105567w-0022-683-E11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K105567w-0022-683-E11C-8409
TTGAATATATCCTCACGTGTGTCGTAAAACGATCCGTTTATTGAATTCTAATCCACGTGT
GGGGAATCTAACACGTACAAAGTTGTAGAGAAAATGTATATCCATAACCCCCTAACGTGT
GAGAATTATGAAAATTATAATCTTTTGGTGTTGAAGACTTCGCTTGCACAATCCGAAGAA
AATATTTGTAAAATATAGCGAAATTTAGATAAAACTATGATTTTTTTAAAAGGTATAATT
ATAAGGAGTCATTAGTTTATTTTGATTTAGAATGTAAAGGAAAAAGATGGAGTTAGTGGG
TATTGGGGATTTGGA
Primers used for PCR AR12 - 8474, show primer details
   
Sequence Name 63-K118446-0022-683-E11C-AR12
GenBank Accession LN511084 [GenBank]
Sequencing primer gene specific primer, AR12: 5'-TAAGAGCAAGAAGGAGACTTTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K118446-0022-683-E11C-AR12
GGAACCCAACGACCTCACTGATTTGATCCGGCGGTTACTGGTGAAGGATCCGAATAGAAG
GTTAGGTTGCCACCGTGGCGCGGCGGAGATAAAAGAGCTCGCTTTTTTCGCGGGTGTGAG
GTGGGATTTGTTAACGGAGGTGTTACGACCTCCGTTTATTCCGTTAAGAGATGACGGAGA
GTTAACGGTAGGTGGGTTTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 23-K118445-0022-683-E11C-8409
GenBank Accession LN496757 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118445-0022-683-E11C-8409
CTTTACAATTGAATATATTCAAACCCACCTACCGTTAACTCTCCGTCATCTCTTAACGGA
ATAAACGGAGGTCGTAACACCTCCGTTAACAAATCCCACCTCACACCCGCGAAAAAAGCG
AGCTCTTTTATCTCCGCCGCGCCACGGTGGCAACCTAACCTTCTATTCGGATCCTTCACC
AGTAACCGCCGGATCAAATCAGTGAGGTCGTTGGGTTTTCCGGCGAACTCGGGTTCCTTC
ATCAACACGTTACGAAAAGTCTCCTTNTTGCTCTTA

Last Updated on 06.12.2018 16:11