Sequences from confirmation amplicon


Confirmation sequences for line 683G12 and hit At4g05020 are shown.
Line ID 683G12
Locus At4g05020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2575655
Primers used for PCR 22ww - 8474, show primer details
   
Sequence Name 48-K118874-0022-683-G12C-22ww
GenBank Accession LN506652 [GenBank]
Sequencing primer gene specific primer, 22ww: 5'-TACCTAGCTAAATGCTTTGACCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K118874-0022-683-G12C-22ww
TTAGGATGAGAGGAGAAGGTCGTCACCGNTTTCCGCCCCTTCAGGTTAGTCCTCCCTAAA
AATTTGAACCAATATCTTTGTCTTATTGTTGATTAAACCAAACCAAAACTGTGACGTAGT
AGATATAGTGTGTAAGCATTGATCCTTAATGTTACATAGTTTTTGAGAATATGGAAGTTG
AGAGGAATTTCCAAAACTCTGTCTCAAGATACTACTGAGTCATGTGCATTGGACCATAAG
GGTGTAGGAAAGATGGAGTTAATTCCTTATATTATTAGGTAAGAATGAAAAAGATAAAGA
GGACGTTAGGAAGCACATTTAGTGAGTTTATGTAGTTTAATCCTAATTTTGATTTGGTTG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGA
   
Sequence Name 08-K118873-0022-683-G12C-8409
GenBank Accession LN489948 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K118873-0022-683-G12C-8409
AATTGAATATATCCAACCAAATCAAAATTAGGATTAAACTACATAAACTCACTAAATGTG
CTTCCTAACGTCCTCTTTATCTTTTTCATTCTTACCTAATAATATAAGGAATTAACTCCA
TCTTTCCTACACCCTTATGGTCCAATGCACATGACTCAGTAGTATCTTGAGACAGAGTTT
TGGAAATTCCTCTCAACTTCCATATTCTCAAAAACTATGTAACATTAAGGATCAATGCTT
ACACACTATATCTACTACGTCACAGTTTTGGTTTGGTTTAATCAACAATAAGACAAAGAT
ATTGGTTCAAATTTTTAGGGAGGACTAACCTGAAGGGGCGGAAACGGTGACGACCTTCTC
CTCTCATCCTAATGGGACCTTCTGGACTCTTCTCACACTCTTCCATACGGTCAAAGCA

Last Updated on 06.12.2018 16:11