Sequences from confirmation amplicon


Confirmation sequences for line 684B05 and hit At1g07240 are shown.
Line ID 684B05
Locus At1g07240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2224587
Primers used for PCR HV05 - 8474, show primer details
   
Sequence Name 15-K031934-022-684-B05C-8409
GenBank Accession LN492858 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K031934-022-684-B05C-8409
TTCAATTGAATATATCCTCACGATATTACCAATTTCTCCATATTGAACATTTTTCTCAAG
GACGAGATTACCCTCACGTGTATCCTGTTGGGCCGGTTCTCAACTTAACGGGCCGTACAA
ATCCGGGTCTAGCTTCGGCCCAATATAAAGAGATGATGAAGTGGCTTGACGAGCAACCAG
ACTCGTCGGTTTTGTTCCTGTGTTTCGGGAGCATGGGAGTCTTCCCTGCACCTCAGATCA
CAGAGATTGCTCACGCGCTCGAGCTTATCGGGTGCAGGTTCATCTGGGCGATCCGTACGA
ACATGGCGGGAGATGGCGATCCTCAGGAGCCGCTTCCAGAAGGATTTGTCGATCGAACAA
TGGGCCGTGGAATTGTGTGTAGTTGGGCTCCACAAGTGGATATCTTGGCCCACAAGGCAA
CAGGTGGATTCGTTTCTCACTGCGGGTGGAATTCCGTCCAAGAGAGTCTATGGTACGGTG
TACCTATTGCAACGTGGCCAATGTATGCGGAGCAACAACTGAACGCATTTGAGATGGTGA
AGGAGTTGGGCTTAGCAGTGGAGAT
   
Sequence Name 16-K031934-022-684-B05C-HV05
GenBank Accession LN493325 [GenBank]
Sequencing primer gene specific primer, HV05: 5'-GTAGTCAAGCCTTATCTCCACTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K031934-022-684-B05C-HV05
ATGCGTTCAGTTGTTGCTCCGCATACATTGGCCACGTTGCAATAGGTACACCGTACCATA
GACTCTCTTGGACGGAATTCCACCCGCAGTGAGAAACGAATCCACCTGTTGCCTTGTGGG
CCAAGATATCCACTTGTGGAGCCCAACTACACACAATTCCACGGCCCATTGTTCGATCGA
CAAATCCTTCTGGAAGCGGCTCCTGAGGATCGCCATCTCCCGCCATGTTCGTACGGATCG
CCCAGATGAACCTGCACCCGATAAGCTCGAGCGCGTGAGCAATCTCTGTGATCTGAGGTG
CAGGGAAGACTCCCATGCTCCCGAAACACAGGAACAAAACCGACGAGTCTGGTTGCTCGT
CAAGCCACTTCATCATCTCTTTATATTGGGCCGAAGCTAGACCCGGATTTGTACGGCCCG
TTAAGTTGAGAACCGGCCCAACAGGATACACGTGAGGGTAATCTCGTCCTTGAGAAAAAT
GTTCAATATGGAGAAATTGGTAATATCGTGAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
Primers used for PCR FS16 - 8474, show primer details
   
Sequence Name 57-K118414-0022-684-B05C-FS16
GenBank Accession LN509546 [GenBank]
Sequencing primer gene specific primer, FS16: 5'-TAGTCTCATCATCATCATCTTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118414-0022-684-B05C-FS16
ATTTCTTCTGCGTTGGTTTGATCGACATCGGCCGTGAGGTAAACCTTCCTTCCTATATCT
TCATGACTTCCAACTTTGGTTTCTTAGGGGTTCTACAGTATCTCCCGGAACGACAACGTT
TGACTCCGTCGGAGTTCGATGAGAGCTCCGGCGAGGAAGAGTTACATATTCCGGCGTTTG
TGAACCGTGTTCCCGCCAAGGTTCTGCCGCCAGGTGTGTTCGATAAACTCTCTTACGGGT
CTCTGGTCAAAATCGGCGAGCGATTACATGAAGCCAAGGGTATTTTGGTTAATTCATTTA
CCCAAGTGGAGCCTTATGCTCAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAAGGTTTATT
   
Sequence Name 09-K118413-0022-684-B05C-8409
GenBank Accession LN490399 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K118413-0022-684-B05C-8409
CATTTACATTGAATATATTGAGCATAAGGCTCCACTTGGGTAAATGAATTAACCAAAATA
CCCTTGGCTTCATGTAATCGCTCGCCGATTTTGACCAGAGACCCGTAAGAGAGTTTATCG
AACACACCTGGCGGCAGAACCTTGGCGGGAACACGGTTCACAAACGCCGGAATATGTAAC
TCTTCCTCGCCGGAGCTCTCATCGAACTCCGACGGAGTCAAACGTTGTCGTTCCGGGAGA
TACTGTAGAACCCCTAAGAAACCAAAGTTGGAAGTCATGAAGATATAGGAAGGAAGGTTT
ACCTCACGGCCGATGTCGATCAAACCAACGCAGAAGAAATCAAGAATCAAGCCGGCGACA
TGACTACTACCACCTCCGGAAGATGATGATATTGAGACT

Last Updated on 06.12.2018 16:11