Sequences from confirmation amplicon


Confirmation sequences for line 684E04 and hit At1g74510 are shown.
Line ID 684E04
Locus At1g74510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28007339
Primers used for PCR CQ80 - 8474, show primer details
   
Sequence Name 31-K107131-0022-684-E04C-CQ80
GenBank Accession LN500187 [GenBank]
Sequencing primer gene specific primer, CQ80: 5'-GGAATTTCTATTGCATTGGAGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K107131-0022-684-E04C-CQ80
TGATCCTGAAGAAGAAGACATGGACATTGATACCTAACATGTTACCTGAAAGAAGCAGTG
GAGGTGGAGGAGATCAAGCAAAAGAGATTGCTGCTGCAACAGCGGCTTCAGAGGCTCCAC
CGCTTGTAGCAGTTGTAAAAGACGAGCTTTATGCTGCGAATTACGCGCAGCAAGAGGTGA
AGAAATACGATAAAAGGCTTAATGTATGGAATAAGGTAGGGAATTTGCCAGAGAGGGCTT
CTTCGATGAACGGTTGGGGAATGGCGTTTAGGGCTTGTGGGGATCAGTTAGTGGTGGTTG
GAGGACCTAGGGCAATAGGAGGAGGGTTTATAGAGATCAATGCTTGTGTCCCAAGTGAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTACGCACGNTTTA
TTA
   
Sequence Name 15-K107130-0022-684-E04C-8409
GenBank Accession LN492997 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K107130-0022-684-E04C-8409
CATTCACTTGGGACACAGCATTGATCTCTATAAACCCTCCTCCTATTGCCCTAGGTCCTC
CAACCACCACTAACTGATCCCCACAAGCCCTAAACGCCATTCCCCAACCGTTCATCGAAG
AAGCCCTCTCTGGCAAATTCCCTACCTTATTCCATACATTAAGCCTTTTATCGTATTTCT
TCACCTCTTGCTGCGCGTAATTCGCAGCATAAAGCTCGTCTTTTACAACTGCTACAAGCG
GTGGAGCCTCTGAAGCCGCTGTTGCAGCAGCAATCTCTTTTGCTTGATCTCCTCCACCTC
CACTGCTTCTTTCAGGTAACATGTTAGGTATCAATGTCCATGTCTTCTTCTTCAGATCAT
AGACTTCACCGCACAATAGCATCTTCGAGTTTCCCTCTCCGATACCTCCAATGCATANAA
AATTC

Last Updated on 06.12.2018 16:11