Sequences from confirmation amplicon


Confirmation sequences for line 684E11 and hit At1g68000 are shown.
Line ID 684E11
Locus At1g68000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25492005
Primers used for PCR HV08 - 8474, show primer details
   
Sequence Name 01-K031934-022-684-E11C-8409
GenBank Accession LN486456 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K031934-022-684-E11C-8409
GAGCCTTTACAATTGAATATATAGAGTTAGCACGGCTTGTCTTCTCGTGATTCTCTCTCA
AATCTACAGGTGAGAATACCTCTCTATATCTTTTTCCGTAGCATCTCTGGAGTTCTACAT
TATTACAGTATATGTAATTTTATTTCATCTTTAAAATAGGCCTAGTTTGGTCTTCCTCTC
ATTGCTGGCTTTAGATATTGCTAGTCATTGGCTGCAGATGTACAGGTACGCTAATTAACT
CTTTCTTGTTTCACCTTGAATTCTTCTCTATACTATTCAAATCTTCAGTTTCTAACTAGG
GACTTCATATTCTACTCGCAGTACGTTTCTAGCAGGGAAGAGCAGCCATAAGGATGTGAA
AGACAGCACAAGCTGGCTTTTTAGACTCTACTATGGAAACCGGATATTTATGTGTTACTG
CTGTGTTTCTTGCGAGGTGACTCAAATTGTCACTTCATTTCCATGTTAATCCTTTGAGAC
GATGTCTTCTTAGACACAAATCTTTTCAAACTTGCAGGTTCTGTATATCATCCTTCTTCT
CATTGCAAAGAACCAATCTGAAAATCTCCTG
   
Sequence Name 02-K031934-022-684-E11C-HV08
GenBank Accession LN486917 [GenBank]
Sequencing primer gene specific primer, HV08: 5'-TCAGGAGATTTTCAGATTGGTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K031934-022-684-E11C-HV08
TGCAGTTTGAAAGATTTGTGTCTAAGAAGACATCGTCTCAAAGGATTAACATGGAAATGA
AGTGACAATTTGAGTCACCTCGCAAGAAACACAGCAGTAACACATAAATATCCGGTTTCC
ATAGTAGAGTCTAAAAAGCCAGCTTGTGCTGTCTTTCACATCCTTATGGCTGCTCTTCCC
TGCTAGAAACGTACTGCGAGTAGAATATGAAGTCCCTAGTTAGAAACTGAAGATTTGAAT
AGTATAGAGAAGAATTCAAGGTGAAACAAGAAAGAGTTAATTAGCGTACCTGTACATCTG
CAGCCAATGACTAGCAATATCTAAAGCCAGCAATGAGAGGAAGACCAAACTAGGCCTATT
TTAAAGATGAAATAAAATTACATATACTGTAATAATGTAGAACTCCAGAGATGCTACGGA
AAAAGATATAGAGAGGTATTCTCACCTGTAGATTTGAGAGAGAATCACGAGAAGACAAGC
CGTGCTAACTCTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
AATCTTTATTTCGACGTGTCTACATTC
Primers used for PCR FS14 - 8474, show primer details
   
Sequence Name 68-K118365-0022-684-E11C-8409
GenBank Accession LN512095 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K118365-0022-684-E11C-8409
TTGAAGCCTGTAGATAAGTAGGTTGATCCTTGCATATATGTAGAAGAGGATTCACCTATC
TGTGACCATGTCAAGAACAGCTCCAAATGTTGAAACTGAAGAAGAAAGAAAGAGAAGTGA
GTCAAGTTTCTAGCTTCCTACATATTCTAGTCATGGCATGTGATGCATGTCTATCACATG
AAACTAAACACTAGCAACATATTGTACTAATCTTGATCAGTAGTAGGATCTTTTGACCAA
CCATACGGAATAAAAATGAGGTTTTATAAAACAAGAGAGAGTTGTTGAACCTTGGTTGAA
TCTACGAGCCACCCAGCCATCCACAGCATCACAACAAAAGCTAGAGACAGCCACATTGAT
CAAAAAGAAGACTGTTAATACCATCACCCAAAACATGAAAAAATCTTAATGACCATCAAA
GAAGAANGTTGCAA

Last Updated on 06.12.2018 16:11