Sequences from confirmation amplicon


Confirmation sequences for line 685A10 and hit At1g13730 are shown.
Line ID 685A10
Locus At1g13730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4711120
Primers used for PCR MG12 - 8474, show primer details
   
Sequence Name 14-K031455-022-685-A10C-MG12
GenBank Accession LN492389 [GenBank]
Sequencing primer gene specific primer, MG12: 5'-TCTTGTATAAGTCACCATCGCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K031455-022-685-A10C-MG12
GTGTTTAGGCCGTCCTGGTTCTGATGGAGAGATGGTTTCTGTCAATCCTTGAAAGTAAGC
TATTGGATGGAGTTGCTTTTTGTGTTCAAACATATGTAAATCTTGTTATTGGAAGCAAAG
TTTCGTTTTTAAGAGTTGTTTAATCTTGTTTTGCAGGCTATCAATGAGCAGATTATGTCC
TTCGATTACGAAATCTCAAAGATTCAGATTTTGACTGCTGATTCCCAAGCATCTTACATG
AATGGAGTTGTGACTCTAGTCACAGGTTTACTAACCGTGAAAGAGGGGCAAAGAATGAGA
TTTTCTCAATCGTTTTTCCTTGTGCCGCTGAATGGAAGCTATTTCGTTCTCAACGATGTC
TTTAGGTATGTTGCTGATGAGATTGTTGAACCAGAAGCTAACAAGAAAGAAGTCGAGGAG
GTTATTCCTCAAGTGGTCCAACCAACAGAGCAGGTAGATGAAGTTGCAGAGCCGGTCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTCA
   
Sequence Name 06-K031455-022-685-A10C-8409
GenBank Accession LN488688 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K031455-022-685-A10C-8409
GAGCCATTTACAATTGAATATATCCTGACCGGCTCTGCAACTTCATCTACCTGCTCTGTT
GGTTGGACCACTTGAGGAATAACCTCCTCGACTTCTTTCTTGTTAGCTTCTGGTTCAACA
ATCTCATCAGCAACATACCTAAAGACATCGTTGAGAACGAAATAGCTTCCATTCAGCGGC
ACAAGGAAAAACGATTGAGAAAATCTCATTCTTTGCCCCTCTTTCACGGTTAGTAAACCT
GTGACTAGAGTCACAACTCCATTCATGTAAGATGCTTGGGAATCAGCAGTCAAAATCTGA
ATCTTTGAGATTTCGTAATCGAAGGACATAATCTGCTCATTGATAGCCTGCAAAACAAGA
TTAAACAACTCTTAAAAACGAAACTTTGCTTCCAATAACAAGATTTACATATGTTTGAAC
ACAAAAAGCAACTCCATCCAATAGCTTACTTTCAAGGATTTGACAGAAACCATCTCTCCA
TCAGAACCAGGACGGCCTAAAACACTATCGTCGAGATAAAACTGATGCACTTGCGATGGT
GACTAATACAAG

Last Updated on 06.12.2018 16:11