Sequences from confirmation amplicon


Confirmation sequences for line 685G11 and hit At5g12920 are shown.
Line ID 685G11
Locus At5g12920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4079754
Primers used for PCR ME42 - 8474, show primer details
   
Sequence Name 20-K110172-0022-685-G11C-ME42
GenBank Accession LN495255 [GenBank]
Sequencing primer gene specific primer, ME42: 5'-CAAGGAAACATGATCAAGTGCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K110172-0022-685-G11C-ME42
CTCCAACCACTGAGGTATGATTTCTGACTTTGAGATCACATGGATATCAGTTATATGGTT
GTTTGTCAAATGAAATTGTTTCTGGTTACAACTCGTGTGACTGCATCATCATGACTTACG
TAGGTTTTCTTCTTGTTAGCTCAATTTATATTTTATATATTAGAAACAAGAAACACAGAT
GTATCGCCGTATTTCTATCTTAGGCAATTGCAAAATTCCCGTTCCAACATGGTTTCCGAA
GAGTGTATTTATTGTTCCCATCACGATTAGAAGAATGGCAGTCAATTATTAATTGAATTG
ACACGGCCATGGTAAGACTTATCAATTTATCATTAGTTTTAGTCTGCTCTACTTAGGCTA
GCCCTGCTAAATATTCATAACCTTTCACCCACATTTCAATTATTTTTCTATTTGTGATTC
AGGTATTGCAAACTAGACAGAAGCTATCGATTATTGGCCGCAAAATTTCTAGAGGTTTAT
CTGATGTTGCCATTACAAGTGATGAGGATTCGAGGTTTGTATTCTTCCTTGTGATGAANA
AACATTTATAGCTTCATTTTACCTACTTTCTTCAGCGACTTTTCTGCATTTGTTTTTAGC
AGAATATTTTCTCCAGATACACTTGGTATGGTTCATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCCNTTTTTTTTCAATT
   
Sequence Name 04-K110171-0022-685-G11C-8409
GenBank Accession LN487965 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K110171-0022-685-G11C-8409
ATCGGACTGAGCCATTTACAATTGAATATGAACCTACCAAGTGTATCTGGAGAAAATATT
CTGCTAAAAACAAATGCAGAAAAGTCGCTGAAGAAAGTAGGTAAAATGAAGCTATAAATG
TTTTTTCATCACAAGGAAGAATACAAACCTCGAATCCTCATCACTTGTAATGGCAACATC
AGATAAACCTCTAGAAATTTTGCGGCCAATAATCGATAGCTTCTGTCTAGTTTGCAATAC
CTGAATCACAAATAGAAAAATAATTGAAATGTGGGTGAAAGGTTATGAATATTTAGCAGG
GCTAGCCTAAGTAGAGCAGACTAAAACTAATGATAAATTGATAAGTCTTACCATGGCCGT
GTCAATTCAATTAATAATTGACTGCCATTCTTCTAATCGTGATGGGAACAATAAATACAC
TCTTCGGAAACCATGTTGGAACGGGAATTTTGCAATTGCCTAAGATAGAAATACGGCGAT
ACATCTGTGTTTCTTGTTTCTAATATATAAAATATAAATTGAGCTAACAAGAAGAAAACC
TACGTAAGTCATGATGATGCAGTCACACGAGTTGTAACCAGAAACAATTTCATTTGACAA
ACAACCATATAACTGATATCCATGTGATCTCAAAGTCAGAAATCATACCTCAGTTGGTTT
GGGAGACATGTAACTGATATCGAAGATAAGCACTTGATC
Primers used for PCR ME42 - 8474, show primer details
   
Sequence Name 51-K027641-022-685-G11C-8409
GenBank Accession LN507384 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K027641-022-685-G11C-8409
CCATTTACATTGAATATGAACCATACCAAGTGTATCTGGAGAAAATATTCTGCTAAAAAC
AAATGCAGAAAAGTCGCTGAAGAAAGTAGGTAAAATGAAGCTATAAATGTTTTTTCATCA
CAAGGAAGAATACAAACCTCGAATCCTCATCACTTGTAATGGCAACATCAGATAAACCTC
TAGAAATTTTGCGGCCAATAATCGATAGCTTCTGTCTAGTTTGCAATACCTGAATCACAA
ATAGAAAAATAATTGAAATGTGGGTGAAAGGTTATGAATATTTAGCAGGGCTAGCCTAAG
TAGAGCAGACTAAAACTAATGATAAATTGATAAGTCTTACCATGGCCGTGTCAATTCAAT
TAATAATTGACTGCCATTCTTCTAATCGTGATGGGAACAATAAATACACTCTTCGGAAAC
CATGTTGGAACGGGAATTTTGCAATTGCCTAAGATAGAAATACGGCGATACATCTGTGTT
TCTTGTTTCTAATATATAAAATATAAATTGAGCTAACAAGAAGAAAACCTACGTAAGTCA
TGATGATGCAGTCACACGAGTTGTAACCAGAAACAATTTCATTTGACAAACAACCATATA
ACTGATATCCATGTGATCTCAAAGTCAGAAATCATACCTCAGTTGGTTTGGGAGACATGT
AACTGATATCGA
   
Sequence Name 52-K027641-022-685-G11C-ME42
GenBank Accession LN507696 [GenBank]
Sequencing primer gene specific primer, ME42: 5'-CAAGGAAACATGATCAAGTGCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K027641-022-685-G11C-ME42
TCTCCAACCAACTGAGGTATGATTTCTGACTTTGAGATCACATGGATATCAGTTATATGG
TTGTTTGTCAAATGAAATTGTTTCTGGTTACAACTCGTGTGACTGCATCATCATGACTTA
CGTAGGTTTTCTTCTTGTTAGCTCAATTTATATTTTATATATTAGAAACAAGAAACACAG
ATGTATCGCCGTATTTCTATCTTAGGCAATTGCAAAATTCCCGTTCCAACATGGTTTCCG
AAGAGTGTATTTATTGTTCCCATCACGATTAGAAGAATGGCAGTCAATTATTAATTGAAT
TGACACGGCCATGGTAAGACTTATCAATTTATCATTAGTTTTAGTCTGCTCTACTTAGGC
TAGCCCTGCTAAATATTCATAACCTTTCACCCACATTTCAATTATTTTTCTATTTGTGAT
TCAGGTATTGCAAACTAGACAGAAGCTATCGATTATTGGCCGCAAAATTTCTAGAGGTTT
ATCTGATGTTGCCATTACAAGTGATGAGGATTCGAGGTTTGTATTCTTCCTTGTGATGAA
AAAACATTTATAGCTTCATTTTACCTACTTTCTTCAGCGACTTTTCTGCATTTGTTTTTA
GCAGAATATTTTCTCCAGATACACTTGGTATGGTTCATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACNAATTACGATCCGTCGTATTTATNGGCGAAAGCAAT
AAACAAATTATTCTAATT

Last Updated on 06.12.2018 16:11