Sequences from confirmation amplicon


Confirmation sequences for line 686A09 and hit At1g65380 are shown.
Line ID 686A09
Locus At1g65380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24287308
Primers used for PCR RE51 - 8474, show primer details
   
Sequence Name 17-K026963-022-686-A09C-8409
GenBank Accession LN493707 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K026963-022-686-A09C-8409
ATTTCCCGGACATGGGGATACTCTTAGGACACTTAATCTTAGTCGGAATAGATTCGTTGG
TTCGATACCCGCCACATTTGTAAGTCTTAAGGAGCTTAGAGAAGTTGTTCTAAGTGAGAA
TAGAGATTTAGGCGGTGTGGTCCCTCATTGGTTTGGTAATTTCTCAATGAATCTTGAGAG
AGTTGATTTTAGTTTCTGCTCGTTTGTTGGAGAACTACCTGAGAGTTTACTGTATTTGAA
GTCTCTCAAGTACTTGAATCTTGAGAGCAATAACATGACTGGTACACTCAGAGATTTTCA
GCAGCCATTGGTGGTTCTTAATCTTGCTTCAAATCAGTTTTCCGGTACGCTACCTTGTTT
CTACGCCTCTCGTCCGTCTCTTAGTATTCTGAATATAGCTGAGAACTCTCTGGTTGGTGG
ATTACCTTCTTGTTTGGGTTCTTTAAAAGAGCTGAGTCATTTGAATCTATCTTTCAATGG
CTTCAACTACGAGATATCTCCTAGGCTTATGTTTTCAGAGAAGCTTGTGATGTTGGACTT
GAGTCACAACGGGTTTTCAGGTCGTCTTCCTAGTAGAATCTCGG
   
Sequence Name 21-K026963-022-686-A09C-RE51
GenBank Accession LN495515 [GenBank]
Sequencing primer gene specific primer, RE51: 5'-ATTTCTCAGTTGTTTCCGAGATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K026963-022-686-A09C-RE51
GACCTGAAAACCCGTTGTGACTCAAGTCCAACATCACAAGCTTCTCTGAAAACATAAGCC
TAGGAGATATCTCGTAGTTGAAGCCATTGAAAGATAGATTCAAATGACTCAGCTCTTTTA
AAGAACCCAAACAAGAAGGTAATCCACCAACCAGAGAGTTCTCAGCTATATTCAGAATAC
TAAGAGACGGACGAGAGGCGTAGAAACAAGGTAGCGTACCGGAAAACTGATTTGAAGCAA
GATTAAGAACCACCAATGGCTGCTGAAAATCTCTGAGTGTACCAGTCATGTTATTGCTCT
CAAGATTCAAGTACTTGAGAGACTTCAAATACAGTAAACTCTCAGGTAGTTCTCCAACAA
ACGAGCAGAAACTAAAATCAACTCTCTCAAGATTCATTGAGAAATTACCAAACCAATGAG
GGACCACACCGCCTAAATCTCTATTCTCACTTAGAACAACTTCTCTAAGCTCCTTAAGAC
TTACAAATGTGGCGGGTATCGAACCAACGAATCTATTCCGACTAAGATTAAGTGTCCTAA
GAGTATCCCCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT

Last Updated on 06.12.2018 16:11