Sequences from confirmation amplicon


Confirmation sequences for line 687C12 and hit At5g06720 are shown.
Line ID 687C12
Locus At5g06720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2078515
Primers used for PCR MK41 - 8474, show primer details
   
Sequence Name 94-K031696-022-687-C12C-8409
GenBank Accession LN515273 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K031696-022-687-C12C-8409
ATTTACAATTGAATACTCATTGTCGATCTTGCTTGACGACACTGGAAGCATCCAGAGCGA
GAAGAACGCTGGTCCGAATGTAAACTCAGCTAGAGGATTCAACGTTGTTGATAATATCAA
GACTGCCCTCGAAAACGCTTGCCCTGGTGTTGTCTCTTGCTCTGACGTTTTAGCCCTTGC
CTCTGAGGCTTCTGTGTCTTTGGTAACTCTACACGTTCTTTGTGGACATATGAAACAAAA
CATAACTAAAAATTACTGTGGTATAATTCTATTTAGTTATTGTTAAGATACGTTTGATCA
TCTTCAGGCAGGAGGGCCATCATGGACTGTATTATTAGGAAGAAGAGATAGTCTCACGGC
CAACCTCGCCGGTGCAAATTCGTCCATTCCTTCTCCCATCGAAAGCCTAAGCAACATCAC
ATTTAAATTTTCGGCTGTCGGGCTTAATACAAACGATCTGGTAGCCTTATCTGGTAAGTT
CATCTAAATGTTTAGTTACTTGCGTCTCAAGTTGAAACAAACCCTAACGCCAACTCTTGT
GGATCGACGTAGGTGCGCATACGTTCGGGCGTGCTCGATGTGGAGTATTCAACAACAGAC
TATTTAATTCCAGCGGG
   
Sequence Name 46-K031696-022-687-C12C-MK41
GenBank Accession LN505766 [GenBank]
Sequencing primer gene specific primer, MK41: 5'-TCCCGCTGAAGTTAAATAGTCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K031696-022-687-C12C-MK41
CCCGACGTAGCGCACCTCNTCGATCCACAAGAGTTGGCGTTAGGGTTTGTTTCAACTTGA
GACGCAAGTAACTAAACATTTAGATGAACTTACCAGATAAGGCTACCAGATCGTTTGTAT
TAAGCCCGACAGCCGAAAATTTAAATGTGATGTTGCTTAGGCTTTCGATGGGAGAAGGAA
TGGACGAATTTGCACCGGCGAGGTTGGCCGTGAGACTATCTCTTCTTCCTAATAATACAG
TCCATGATGGCCCTCCTGCCTGAAGATGATCAAACGTATCTTAACAATAACTAAATAGAA
TTATACCACAGTAATTTTTAGTTATGTTTTGTTTCATATGTCCACAAAGAACGTGTAGAG
TTACCAAAGACACAGAAGCCTCAGAGGCAAGGGCTAAAACGTCAGAGCAAGAGACAACAC
CAGGGCAAGCGTTTTCGAGGGCAGTCTTGATATTATCAACAACGTTGAATCCTCTAGCTG
AGTTTACATTCGGACCAGCGTTCTTCTCGCTCTGGATGCTTCCAGTGTCGTCAAGCAAGA
TCGACAATGAGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATNTCGACGTGTCTACATTCC

Last Updated on 06.12.2018 16:11