Sequences from confirmation amplicon


Confirmation sequences for line 687F10 and hit At3g04420 are shown.
Line ID 687F10
Locus At3g04420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1172996
Primers used for PCR CQ84 - 8474, show primer details
   
Sequence Name 40-K105634-0022-687-F10C-CQ84
GenBank Accession LN503709 [GenBank]
Sequencing primer gene specific primer, CQ84: 5'-CGTTCACAAGAAGAGGGATTAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K105634-0022-687-F10C-CQ84
AGAAGAAGAGAAATCTCTCTCACGTCACCCTTAAACATAACTTTACATATTGTATATGTC
ATCATCATCTGTAAAAAACATTCAGTCAAATCACAATCACCCGATACACGAAAACGAGCA
ATCAAATCACGGTAAAGAGCAAAAACAGAGGGAAAAAAAACCTGAGTAGGAGAGAAAGCA
TGGTACTCGTGCATAACCCAGTCGGATTTGGATCCACCAAGTTCCTTACTCATGTAAAAC
ATCAAAACCTTTTTCTCACCGATCTCTTGGCGGTTACGCAAGATAGGCTTTGGTTTGCCA
GTAATCTTCCAGTAACCAGATTTCGTTTTTCTAATTTGTTGATCACCTCTGCCATATTTG
TTCTCCTTAGGGCTGAAGAAACACCAAGACTCATCTTTCAGTTTGATCCTAGACTGGCCT
GCCCAAAAAGAAAAACCCTAAACTTAAATGGCTATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 56-K105635-0022-687-F10C-8409
GenBank Accession LN509102 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K105635-0022-687-F10C-8409
CTTTACATTGAATATAGCCATTTAAGTTTAGGGTTTTTCTTTTTGGGCAGGCCAGTCTAG
GATCAAACTGAAAGATGAGTCTTGGTGTTTCTTCAGCCCTAAGGAGAACAAATATGGCAG
AGGTGATCAACAAATTAGAAAAACGAAATCTGGTTACTGGAAGATTACTGGCAAACCAAA
GCCTATCTTGCGTAACCGCCAAGAGATCGGTGAGAAAAAGGTTTTGATGTTTTACATGAG
TAAGGAACTTGGTGGATCCAAATCCGACTGGGTTATGCACGAGTACCATGCTTTCTCTCC
TACTCAGGTTTTTTTTCCCTCTGTTTTTGCTCTTTACCGTGATTTGATTGCTCGTTTTCG
TGTATCGGGTGATTGTGATTTGACTGAATGTTTTTTACAGATGATGATGACATATACAAT
ATGTAAAGTTATGTTTAAGGGTGACGTGAGAGAGATTTCTTCTTCTTCTGCTTCTTATGG
TAGTGAAATTGAGCAGAGTCGTGACTCTTTAATCCCTCTCTTTTGTGAACG
Primers used for PCR 15ah - 3144, show primer details
   
Sequence Name 08-K106400-0022-687-F10C-3144
GenBank Accession LN489742 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106400-0022-687-F10C-3144
TCCACTTCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGACT
ACGGATACAAGGTAACTCCCAAGGGTCGAAGCTACGGATAGTGACTGTGCTAATGACTCG
ATCAACATGACTCGTGTCCCTATCGGAGTTTTTTGGTCGGAGGTAATCGACGACGATCTC
CTTGTCGGTCGGACGGAATCTTAAACCCACCGGATTCTCCATTTCTCGAACCAGCGATTA
CGAAGAGAAAAAGTAAAAGAAAAGATCAAAAAAAATTGCAGTAGAGAGTTTTGGCCATGG
TTCTGCAACTTAGATTGGAGAAGAAAGAGATCGAATAGGGTTTTATGTATTTATATGCGC
AGATTCTAAATTTCAAAAATAAATATAAATAATAAACAATTGAAAAATAGTAAATTTATT
AAATAATGGACGTTAAGACATTCAAAGGGTTAAGTTTCTTTGGGATGGTTTTTAATACG
   
Sequence Name 24-K106401-0022-687-F10C-15ah
GenBank Accession LN497004 [GenBank]
Sequencing primer gene specific primer, 15ah: 5'-CGTATTAAAACCATCCCAAAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K106401-0022-687-F10C-15ah
ACGTCATTATTAATAAATTTACTATTTTTCAATTGTTTATTATTTATATTTATTTTTGAA
ATTTAGAATCTGCGCATATAAATACATAAAACCCTATTCGATCTCTTTCTTCTCCAATCT
AAGTTGCAGAACCATGGCCAAAACTCTCTACTGCAATTTTTTTTGATCTTTTCTTTTACT
TTTTCTCTTCGTAATCGCTGGTTCGAGAAATGGAGAATCCGGTGGGTTTAAGATTCCGTC
CGACCGACAAGGAGATCGTCGTCGATTACCTCCGACCAAAAAACTCCGATAGGGACACGA
GTCATGTTGATCGAGTCATTAGCACAGTCACTATCCGTAGCTTCGACCCTTGGGAGTTAC
CTTGTATCCGTAGTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATC
CCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGC
GAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAATCCAATCCA

Last Updated on 06.12.2018 16:11