Sequences from confirmation amplicon


Confirmation sequences for line 687H01 and hit At5g38386 are shown.
Line ID 687H01
Locus At5g38386 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15364277
Primers used for PCR CQ86 - 8474, show primer details
   
Sequence Name 07-K107108-0022-687-H01C-8409
GenBank Accession LN489295 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K107108-0022-687-H01C-8409
ATTTACAATTGAATATAGCCATTTCTCAATTGAATCGATGCCAGTGTTCAAAAACCTCAA
ATCTTTATCTATCAAGAGTGTTGAGAGTCGAGGATGGCAAGCAATGCCAGTTCTTCTAAG
GAACTGTCCACATTTAGAAACTCTAGTCCTTGAGGTATAGAAATAACTAATTTACCAAAC
ATGTACAAAGATAGAGTTCACACATTGAAACCTTAAATGTCTCCTTTATGCCTTCAGGCT
CTCTTGCACCATGTAACTGATAAATGCGGGGATGCTTGTGCCTGCGTCTCTCGGGAGGAG
AAAGGTCGTTCGCTCAAATCTTGTCCAGTAAAAGTGTTAGAGATTAAAGAGTTTCAAGGA
ACAATGAAAGAGATGCACATGATAAAACATTTCCTGGACTATCTTCCGTGTTTGAAGGAG
ATGAAAATCTCCTATATGAAAAAGAATGATCATACTACACAGTTCAGAGTCATCCCTCAA
GTTATTGCAGAGATGGTGGAACACTACAACAAGTTATCTAATTG
   
Sequence Name 23-K107113-0022-687-H01C-CQ86
GenBank Accession LN496578 [GenBank]
Sequencing primer gene specific primer, CQ86: 5'-CAAGCTGGACATTGCAATTAGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K107113-0022-687-H01C-CQ86
CATAACTTGAGGGATGACTCTGAACTGTGTAGTATGATCATTCTTTTTCACTATAGGAGA
TTTTCATCTCCTTCAAACACGGAAGATAGTCCAGGAAATGTTTTATCATGTGCATCTCTT
TCATTGTTCCTTGAAACTCTTTAATCTCTAACACTTTTACTGGACAAGATTTGAGCGAAC
GACCTTTCTCCTCCCGAGAGACGCAGGCACAAGCATCCCCGCATTTATCAGTTACATGGT
GCAAGAGAGCCTGAAGGCATAAAGGAGACATTTAAGGTTTCAATGTGTGAACTCTATCTT
TGTACATGTTTGGTAAATTAGTTATTTCTATACCTCAAGGACTAGAGTTTCTAAATGTGG
ACAGTTCCTTAGAAGAACTGGCATTGCTTGCCATCCTCGACTCTCAACACTCTTGATAGA
TAAAGATTTGAGGTTTTTGAACACTGGCATCGATTCAATTGAGAAATGGCTATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
Primers used for PCR CQ85 - 8474, show primer details
   
Sequence Name 08-K107108-0022-687-H01C-oriL
GenBank Accession LN489754 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K107108-0022-687-H01C-oriL
TCATTGAATATATCCTGATAACAAGACAAACCATAATTAGACTTTTGCTTATTTGTTAAA
AATAGGACTTGAACAAACATGACTGCCATGCTCCATAAATTGAGACGAAGTGCTGGTTAT
CTTTCATTTATTTTACCAAGACCAGCTAATAGAGAAAAGAACAAAAACACTAACCTCAAG
AGTATCACGAGAGAAATAGAGATACCGAACATTTCGAATGCTATTCATAAGCTTCCCCAC
ATTTTCATATCGGAGAACATGCATGATTGTACTCATCATCCTCTAACAAATCATTGTTTG
GCGCTCTTACTCGGCTGATATCGTCTTCAGGTACAAAAGGGCTGAT
   
Sequence Name 24-K107113-0022-687-H01C-CQ85
GenBank Accession LN497014 [GenBank]
Sequencing primer gene specific primer, CQ85: 5'-AATCAGCCTTTTTGTACCTGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K107113-0022-687-H01C-CQ85
GCAACATGATTTGTTGAGGATGATGAGTACATCATGCATGTTCTCCGATATGAAAATGTG
GGGAAGCTTATGAATAGCATTCGAAATGTTCGGTATCTCTATTTCTCTCGTGATACTCTT
GAGGTTAGTGTTTTTGTTCTTTTCTCTATTAGCTGGTCTTGGTAAAATAAATGAAAGATA
ACCAGCACTTCGTCTCAATTTATGGAGCATGGCAGTCATGTTTGTTCAAGTCCTATTTTT
AACAAATAAGCAAAAGTCTAATTATGGTTTGTCTTGTTATCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTT
ATT

Last Updated on 06.12.2018 16:11