Sequences from confirmation amplicon


Confirmation sequences for line 688C08 and hit At1g18520 are shown.
Line ID 688C08
Locus At1g18520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6375535
Primers used for PCR KQ44 - 8474, show primer details
   
Sequence Name 55-K101565-0022-688-C08C-8409
GenBank Accession LN508761 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K101565-0022-688-C08C-8409
TCGGACATGAGCCATTTACAATTGATAAAACGCATCAGCGATCTGACTAACACGACCATC
ACTCAAATCATCACAAACGTTAGCCTCAGCCAAACAAGACCTTATCCCAACCCATCTCTT
CCCACCAACGAACCCATTAAGCCACGTCGAGAAATCCACCGTCCGGTACTCTTTATACCC
TCGACCGGACACCACACGACCGGCTCCTTTGTTGGTCACAAAGAAGAGAAATATGGAGAA
AATCATCAATGCAACAATGCCCCCAAACAATATGATCAAGTAGGAAACCATTGCCAAATT
CTCCTTGAAACAAGATCCAATCACTCCGAGCAAAGACACCAAGAAGAGGATGAGTCCGGT
CGTGAGAAGTGGTATCCGAATGGCAGATTCACAATCAGTGACGCCTTGGTGAACGAACAT
GTAAATCGAATAACCAATGGCCGAAGCGCCCACTAACATCACCAATGTGTTTGCTAGACC
AACCATGAAATTGCTAACTCGAAACATTTTTGGAAATTTGCTTTCTCCAAATGATAAACT
CGATNAACCTGATGGGATTTT
   
Sequence Name 55-K101578-0022-688-C08C-KQ44
GenBank Accession LN508762 [GenBank]
Sequencing primer gene specific primer, KQ44: 5'-TAAAATCCGATCAGGTTAATCGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K101578-0022-688-C08C-KQ44
ATTTCCAAATGTTTCGAGTTGCAATTTCATGGTTGGTCTAGCAAACACATTGGTGATGTT
AGTGGGCGCTTCGGCCATTGGTTATTCGATTTACATGTTCGTTCACCAAGGCGTCACTGA
TTGTGAATCTGCCATTCGGATACCACTTCTCACGACCGGACTCATCCTCTTCTTGGTGTC
TTTGCTCGGAGTGATTGGATCTTGTTTCAAGGAGAATTTGGCAATGGTTTCCTACTTGAT
CATATTGTTTGGGGGCATTGTTGCATTGATGATTTTCTCCATATTTCTCTTCTTTGTGAC
CAACAAAGGAGCCGGTCGTGTGGTGTCCGGTCGAGGGTATAAAGAGTACCGGACGGTGGA
TTTCTCGACGTGGCTTAATGGGTTCGTTGGTGGGAAGAGATGGGTTGGGATAAGGTCTTG
TTTGGCTGAGGCTAACGTTTGTGATGATTTGAGTGATGGTCGTGTTAGTCAGATCGCTGA
TGCGTTTTATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATAATCCGCAGCGTTA

Last Updated on 06.12.2018 16:11