Sequences from confirmation amplicon


Confirmation sequences for line 688H09 and hit At5g57110 are shown.
Line ID 688H09
Locus At5g57110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23114902
Primers used for PCR BK27 - 8474, show primer details
   
Sequence Name 80-K104542-0022-688-H09C-BK27
GenBank Accession LN513950 [GenBank]
Sequencing primer gene specific primer, BK27: 5'-GGGTATCAAAGAAGGATGGTATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K104542-0022-688-H09C-BK27
TTGTGATTGTTGTGACGGGTAATTTAACTTGTTTCACTTTTTTCTTTTTCTTTTTAGGTG
TCTACAGACTTGTTTCACTTAATATAATAATTTTGATAATGACTCTTACTGTGATTTTGA
TGCTTTCTTCTCCTCTGCAGCTGTCAGTGACTACAAACAGTCGCTCCAGTTTCAAAACTT
GAATGATGAAAAGAGAAACATACATCTGGAGGTATAAATTTTCCCCAATGATACTTTTCC
TCATACTCTCTTTTTCTTATTTCCTTCTTAGTTACACTACTCTTTCTGTTGACTGCTGCA
CAGGTGTTAAGAGGTGGAAGACGAGTGGAGATTTCGATCTATGACATAGTGGTGGGTGAT
GTCATTCCTCTCAACATTGGCAATCAGGTGAGTGGTTTATTCTCCTTTTAATTTTGTTTT
TGTCCATATTACCTTGTCTAATCGAAGTTTAAATTTATATCTAGGTTCCTGCTGATGGAG
TACTGATATCTGGCCACTCTCTCGCCCTTGATGAATCTAGCATGACTGGAGAGAGCAAAA
TTGTGTGTTTTTGTTTCCTGTCATAGTAAAATTGTTGATTTTCTCGCTTCTTCTTGGTAT
AACTTTCTATGTGTCAATTTCCCTTTCCTTGTCAGGTTAACAAAGACGCTAACAAGGATC
AGCAATGTCCTTGTCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCC
   
Sequence Name 96-K104543-0022-688-H09C-8409
GenBank Accession LN515390 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K104543-0022-688-H09C-8409
CATTTACAATTGGACAAGGACATTGCTGATCCTTGTTAGCGTCTTTGTTAACCTGACAAG
GAAAGGGAAATTGACACATAGAAAGTTATACCAAGAAGAAGCGAGAAAATCAACAATTTT
ACTATGACAGGAAACAAAAACACACAATTTTGCTCTCTCCAGTCATGCTAGATTCATCAA
GGGCGAGAGAGTGGCCAGATATCAGTACTCCATCAGCAGGAACCTAGATATAAATTTAAA
CTTCGATTAGACAAGGTAATATGGACAAAAACAAAATTAAAAGGAGAATAAACCACTCAC
CTGATTGCCAATGTTGAGAGGAATGACATCACCCACCACTATGTCATAGATCGAAATCTC
CACTCGTCTTCCACCTCTTAACACCTGTGCAGCAGTCAACAGAAAGAGTAGTGTAACTAA
GAAGGAAATAAGAAAAAGAGAGTATGAGGAAAAGTATCATTGGGGAAAATTTATACCTCC
AGATGTATGTTTCTCTTTTCATCATTCAAGTTTTGAAACTGGAGCGACTGTTTGTAGTCA
CTGACAGCTGCAGAGGAGAAGAAAGCATCAAAATCACAGTAAGAGTCATTATCAAAATTA
TTATATTAAGTGAAACAAGTCTGTAGACACCTAAAAAGAAAAAGAAAAAAGTGAAACAAG
TTAAATTACCCGTCACAACAATCACAAGAATCACAGCAAATGCAATGCTTCCTCCATCAT
ACCATCCT

Last Updated on 06.12.2018 16:11