Sequences from confirmation amplicon


Confirmation sequences for line 689A11 and hit At4g30160 are shown.
Line ID 689A11
Locus At4g30160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14754849
Primers used for PCR HV57 - 8474, show primer details
   
Sequence Name 71-K101271-0022-689-A11C-8409
GenBank Accession LN512521 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K101271-0022-689-A11C-8409
CGGACTGAGCCTTTACATTGATCTTTTGATCGATCTTTTGTGACCAGACAACGGCGTTAA
AAACTGGTGCATTGCGCCATGATATCCATTACTGGCTTGGTAAAGATACCTCTCAGGTAA
GACCCTGTTATTATTTCCTTTCTTTTTTTACTTTCGTAGAATGACGGAGAAGTTTGAGAG
AAACTGATGATTTTTCTCAAAAACAACTAGGATGAAGCCGGTACTGCTGCAGTTAAGACA
GTTGAATTAGATGCTGCTCTAGGAGGTCGTGCAGTGCAGTATCGGGAAGTTCAAGGCCAC
GAGACTGAGAAATTCTTGTCTTATTTTAAGCCATGTATCATTCCTCAAGAAGGTGGAGTA
GCATCAGGATTCAAGCATGTCGTAGCTGAAGAACATTATACCCGC
   
Sequence Name 71-K101277-0022-689-A11C-HV57
GenBank Accession LN512522 [GenBank]
Sequencing primer gene specific primer, HV57: 5'-AGCGGGTAATATGTTCTTCAGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K101277-0022-689-A11C-HV57
CTTCTTGAGGATGATACATGGCTTAAATAAGACAAGAATTTCTCAGTCTCGTGGCCTTGA
ACTTCCCGATACTGCACTGCACGACCTCCTAGAGCAGCATCTAATTCAACTGTCTTAACT
GCAGCAGTACCGGCTTCATCCTAGTTGTTTTTGAGAAAAATCATCAGTTTCTCTCAAACT
TCTCCGTCATTCTACGAAAGTAAAAAAAGAAAGGAAATAATAACAGGGTCTTACCTGAGA
GGTATCTTTACCAAGCCAGTAATGGATATCATGGCGCAATGCACCAGTTTTTAACGCCGT
TGTCTGGTCACAAAAGATCGATCAAAAGATCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC

Last Updated on 06.12.2018 16:11