Sequences from confirmation amplicon


Confirmation sequences for line 690A10 and hit At5g38435 are shown.
Line ID 690A10
Locus At5g38435 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15388432
Primers used for PCR 30xp - 8474, show primer details
   
Sequence Name 37-K119512-0022-690-A10C-30xp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 30xp: 5'-GGATTATCCGTAGGGTTGAGTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K119512-0022-690-A10C-30xp
TCGGAACAAGTCTTGAAAGTAAATTGCATATCAAATGATGACAATCTAGGCTTCCACTTT
TTGCGCCATGGACAAACCTACGATTTTAGTTTCCACGATAGTCTTTTCAAATCAGAATTC
TATTGTGACCTATGGCAAGGCCCTAATTTCAAGTTGCATGCAGCATTTACGGCATATGAA
GGTGGTGGTTTGATAGTTCATTATGGTAAAAAGAACTTTTGGGATGTTAGAGATGATGGG
ATCTATTTTAATGTCCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCACAACGTTATT
   
Sequence Name 05-K119511-0022-690-A10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K119511-0022-690-A10C-8409
CATTTACATTGAATAATCCTGATGGACATTAAAATAGATCCCATCATCTCTAACATCCCA
AAAGTTCTTTTTACCATAATGAACTATCAAACCACCACCTTCATATGCCGTAAATGCTGC
ATGCAACTTGAAATTAGGGCCTTGCCATAGGTCACAATAGAATTCTGATTTGAAAAGACT
ATCGTGGAAACTAAAATCGTAGGTTTGTCCATGGCGCAAAAAGTGGAAGCCTAGATTGTC
ATCATTTGATATGCAATTTACTTTCAAGACTTTGTTCCGACCAAGAGAATTCGTAAAGAT
GACAGAGTTTTTCTCATTCCACTTTCCGCTACTCAACCCTCGAGATAATC

Last Updated on 06.12.2018 16:11