Sequences from confirmation amplicon


Confirmation sequences for line 690B06 and hit At1g47900 are shown.
Line ID 690B06
Locus At1g47900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17647550
Primers used for PCR 57by - 8474, show primer details
   
Sequence Name 83-K108479-0022-690-B06C-57by
GenBank Accession LN514324 [GenBank]
Sequencing primer gene specific primer, 57by: 5'-CAGTATGAAAATGCACAAGTCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K108479-0022-690-B06C-57by
ACGAAATATTCTCCAGTATTTTGATCACATTTCATCTCATGTACACATACACACAAGTTT
CTCCCCACTTTTATTTTTAAAGGCTTTAATTAATAACCTGATTTGGAGGAGAAGAACCTG
CTTATTCCTCTAGAGGCTTTCTCTGGGGTCGCGTTAGTAGATGAACCTGACCTTGAGAGC
CTGGAACCCACTCGTGATGGCGATGTCGTCGTATCGCTAGTCTCTGAATCAGAGGGGCAT
TTTGGAGACTCCATAAATCTAGGAACCTCGTTAACCGAATCTCCTTCGTCTACTACGGCA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTA
   
Sequence Name 71-K108478-0022-690-B06C-8409
GenBank Accession LN512574 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K108478-0022-690-B06C-8409
CATTTACATTGAATTGCCGTAGTAGACGAAGGAGATTCGGTTAACGAGGTTCCTAGATTT
ATGGAGTCTCCAAAATGCCCCTCTGATTCAGAGACTAGCGATACGACGACATCGCCATCA
CGAGTGGGTTCCAGGCTCTCAAGGTCAGGTTCATCTACTAACGCGACCCCAGAGAAAGCC
TCTAGAGGAATAAGCAGGTTCTTCTCCTCCAAATCAGGTTATTAATTAAAGCCTTTAAAA
ATAAAAGTGGGGAGAAACTTGTGTGTATGTGTACATGAGATGAAATGTGATCAAAATACT
TGGAGAATATTTTCGTTTGGGATCTTTTTGTTACTTACCTGCTTATCAAACGACTTGTGC
ATTTTCATACTG

Last Updated on 06.12.2018 16:11