Sequences from confirmation amplicon


Confirmation sequences for line 690H09 and hit At1g69360 are shown.
Line ID 690H09
Locus At1g69360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26073001
Primers used for PCR 10hd - 8474, show primer details
   
Sequence Name 43-K112477-0022-690-H09C-10hd
GenBank Accession LN504887 [GenBank]
Sequencing primer gene specific primer, 10hd: 5'-TTTTGTCCATTGTTACTTGCTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K112477-0022-690-H09C-10hd
TAATTGTGAATCGGATTATTTCGAAAGCTTTATGTTAATAGCACTGTTGGGGGAGTACCT
GCAGGGCACCTATGTCGCCTTGGTCTTCTGGTTGCATTTCCCCACCCTGTAAGGCTCCTA
TCTTCCAGTCCCACCATTTGTACACTACTGTTATTCATTGGCTGTGCCAAAGAGGAGCAA
ACCGGAGCTCTGTCAATGTTGCTGACCAGTCGTTTTCTTCCTCGGTTTGAGCTCCTTCGG
GCCATTCCTGAGTTCCAGTTGTAGCCTGTGGCTTTCATAAGTCCCCCAAACATCTGAAGA
TCTTCGGTTACTTCGAGCCTAGACAGAGAAGCTAGCCCAGGGAGAATATCCCTTTGGAAA
TCCCGCTTTGGTCTTCCTCGTCTCGCTTGTCCTCTTCTTGGCCTATTACTTGTGATACCC
ATAGAACCCGTCCCATCAAATTTCAGATATTCAGGGATTAAAGGCTTTGGCATGTAGTCT
TCTTCTTTGGTCTGTGTTAAGTTCAGGGTCAGCAATGAGTATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATT
A
   
Sequence Name 20-K112476-0022-690-H09C-8409
GenBank Accession LN495273 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K112476-0022-690-H09C-8409
TTGAATATATACTCATTGCTGACCCTGAACTTAACACAGACCAAAGAAGAAGACTACATG
CCAAAGCCTTTAATCCCTGAATATCTGAAATTTGATGGGACGGGTTCTATGGGTATCACA
AGTAATAGGCCAAGAAGAGGACAAGCGAGACGAGGAAGACCAAAGCGGGATTTCCAAAGG
GATATTCTCCCTGGGCTAGCTTCTCTGTCTAGGCTCGAAGTAACCGAAGATCTTCAGATG
TTTGGGGGACTTATGAAAGCCACAGGCTACAACTGGAACTCAGGAATGGCCCGAAGGAGC
TCAAACCGAGGAAGAAAACGACTGGTCAGCAACATTGACAGAGCTCCGGTTTGCTCCTCT
TTGGCACAGCCAATGAATAACAGTAGTGTACAAATGGTGGGACTGGAAGATAGGAGCCTT
ACAGGGTGGGGAAATGCAACCAGAAGACCAAGGCGACATAGGTGCCCTGCAGGTACTCCC
CCAACAGTGCTATTAACATAAAGCTTTTCGAAATAAATCCGATTTCACAATTTGAGGAAC
ATTATAGAGGGAAAAGAACCAGAGCAAGTAACAATGGACAAA

Last Updated on 06.12.2018 16:11