Sequences from confirmation amplicon


Confirmation sequences for line 692B09 and hit At4g32640 are shown.
Line ID 692B09
Locus At4g32640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15742736
Primers used for PCR NA42 - 8474, show primer details
   
Sequence Name 01-K101562-0022-692-B09C-8409
GenBank Accession LN486512 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K101562-0022-692-B09C-8409
ATTTACATTGAATATATCCTGGAACTCAAATGCTTTAGCCGACAATATGCAGAACTTGAG
CTTGAATCGACCACCTCCGATGTGGCCCGGAATTTTGCTGGCTGTTGGGTCTAGGTGCCC
CTGGAGGCACAGGAGCAACCATGCTTGGATACGAGATGGAGATCAAATCCTAAAACTTCC
TCCACAATCTAAAGTCTTGCGAAAATCAACAAATTCAAGTAATCAATCAGCAAATGCCAC
AATTAAAGAAATTGAAGATAAAAACACTGACACACTAACAATGATCCGATCAATCACACA
GACTTGGTGGCATAANCTACACA
   
Sequence Name 01-K101571-0022-692-B09C-NA42
GenBank Accession LN486513 [GenBank]
Sequencing primer gene specific primer, NA42: 5'-TTGTGTAGTTACTGCCACCAAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K101571-0022-692-B09C-NA42
GTGTTTTATCTTCATTTCTTTAATTGTGGCATTTGCTGATTGATTACTTGAATTTGTTGA
TTTTCGCAAGACTTTAGATTGTGGAGGAAGTTTTAGGATTTGATCTCCATCTCGTATCCA
AGCATGGTTGCTCCTGTGCCTCCAGGGGCACCTAGACCCAACAGCCAGCAAAATTCCGGG
CCACATCGGAGGTGGTCGATTCAAGCTCAAGTTCTGCATATTGTCGGCTAAAGCATTTGA
GTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCA
Primers used for PCR NA43 - 8474, show primer details
   
Sequence Name 02-K101562-0022-692-B09C-OriL
GenBank Accession LN486968 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K101562-0022-692-B09C-OriL
ATTGAATATATCCTGGTTCACAAGGGAACTCAAATGCTTTAGCCGACAATATGCAGAACT
TGAGCTTGAATCGACCACCTCCGATGATGCCTGGCTCAGGTCCTAGACCACCTCCTCCTT
TTGGTCAGTCACCACAGCCTTTCCCTCAGCAATCCCCTTCTTATGGAGCTCCGCAACGTG
GGCCTTCACCCATGTCCCGGCCTGGACCTCCAGCTGGCATGGCGAGACCAGGTGGTCCGC
CTCCTGTATCACAACCAGCTGGGTTTCAGTCAAATGTACCCTTGAACAGACCCACTGGTC
CACCTTCTCGTCAGCCATCATTTGGGTCTAGACCTTCAATGCCTGGAGGACCAGTGGCGC
AGCCTGCCGCCTCTTCTAGTGGTTTTCCAGCTTTTGGTCCATCAGGTAGTGTAGCTGCAG
GTCCTCCTCCAGGATCTCGCCCAATGGCATTTGGTTCACCTCCACCTGTCGGATCTGGCA
TGTCTATGCCTCCCTCAGGCATGATTGGTGGTCCAGTCAGTAATGGGCATCAAATGGTTG
GTTCAGGGGGATTTCCGAGGGGAACCCAGTTCCCCGGTGCTGCTGTTACAACTCCACAGG
CACCCTATGTGCGGCCTCCTTCAGCACCTTACGCTCGGACTCCTCCGCAGCCACTAGGTT
CACATTCTCTTTCCGGAAATCCACCATTAATC
   
Sequence Name 02-K101571-0022-692-B09C-NA43
GenBank Accession LN486969 [GenBank]
Sequencing primer gene specific primer, NA43: 5'-CAGTAAACGGAGTTAATGGTGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K101571-0022-692-B09C-NA43
GACTAGTGGCTGCGGAGGAGTCCGAGCGTAAGGTGCTGAAGGAGGCCGCACATAGGGTGC
CTGTGGAGTTGTAACAGCAGCACCGGGGAACTGGGTTCCCCTCGGAAATCCCCCTGAACC
AACCATTTGATGCCCATTACTGACTGGACCACCAATCATGCCTGAGGGAGGCATAGACAT
GCCAGATCCGACAGGTGGAGGTGAACCAAATGCCATTGGGCGAGATCCTGGAGGAGGACC
TGCAGCTACACTACCTGATGGACCAAAAGCTGGAAAACCACTAGAAGAGGCGGCAGGCTG
CGCCACTGGTCCTCCAGGCATTGAAGGTCTAGACCCAAATGATGGCTGACGAGAAGGTGG
ACCAGTGGGTCTGTTCAAGGGTACATTTGACTGAAACCCAGCTGGTTGTGATACAGGAGG
CGGACCACCTGGTCTCGCCATGCCAGCTGGAGGTCCAGGCCGGGACATGGGTGAAGGCCC
ACGTTGCGGAGCTCCATAAGAAGGGGATTGCTGAGGGAAAGGCTGTGGTGACTGACCAAA
AGGAGGAGGTGGTCTAGGACCTGAGCCAGGCATCATCGGAGGTGGTCGATTCAAGCTCAA
GTTCTGCATATTGTCGGCTAAAGCATTTGAGTTCCCTTGTGAACCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11