Sequences from confirmation amplicon


Confirmation sequences for line 692C06 and hit At4g17040 are shown.
Line ID 692C06
Locus At4g17040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9589296
Primers used for PCR JV45 - 8474, show primer details
   
Sequence Name 34-K101326-0022-692-C06C-8409
GenBank Accession LN501349 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K101326-0022-692-C06C-8409
CCATTTACATTGAATAATCCTGTAATTGAACCTGAAACGTCATCCCTATTTCTTCTTTGT
TGCAGAGAGAGAGAAAGAGTGAAAGTCCCTGTGACCCCGAAACGATAACGATCTGTCTCA
AATCGGGCTTCATAACATTATTTATGGGCTCAACAAATAGGCCCAATAGTTGTAGTTTTT
TTTCTCTGAAAATATTCCGGTTTCGACTATAATAGTAACCAAATCAAACCGAAGTAAAAC
CGGGAATTTCTCATTTTTCGAAAACGTATAATTTAATATGAGGCTACCAAATAAATAATC
TACTGGCCACTGCAGAGAATCATCTGTCGTGTTCTGTTTGTTCTCACTTCTCACTTCTCA
GATTTTTATAAACATGAATGAAACCTGAATTGTTCCACTAACTCTCAGCAAAAATGCGAT
CACTTTAC
   
Sequence Name 34-K101332-0022-692-C06C-JV45
GenBank Accession LN501350 [GenBank]
Sequencing primer gene specific primer, JV45: 5'-AAGTAAAGTGATCGCATTTTTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K101332-0022-692-C06C-JV45
TCATGTTTATAAAATCTGAGAAGTGAGAAGTGAGAACAAACAGAACACGACAGATGATTC
TCTGCAGTGGCCAGTAGATTATTTATTTGGTAGCCTCATATTAAATTATACGTTTTCGAA
AAATGAGAAATTCCCGGTTTTACTTCGGTTTGATTTGGTTACTATTATAGTCGAAACCGG
AATATTTTCAGAGAAAAAAAACTACAACTATTGGGCCTATTTGTTGAGCCCATAAATAAT
GTTATGAAGCCCGATTTGAGACAGATCGTTATCGTTTCGGGGTCACAGGGACTTTCACTC
TTTCTCTCTCTCTGCAACAAAGAAGAAATGGAGATGACGTTTCAGGTTCAATTACAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCA

Last Updated on 06.12.2018 16:11