Sequences from confirmation amplicon


Confirmation sequences for line 692D05 and hit At2g33240 are shown.
Line ID 692D05
Locus At2g33240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14091425
Primers used for PCR 42bk - 8474, show primer details
   
Sequence Name 85-K107706-0022-692-D05C-42bk
GenBank Accession LN514552 [GenBank]
Sequencing primer gene specific primer, 42bk: 5'-CCTTACCTTGAGCTGTTCATTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K107706-0022-692-D05C-42bk
CTCACTCCTTACAGTTCTTCAATTCTAGTTGCATATCTTGTAAAGCAGACCGCTACTTCA
AAATCTCCTCACTTTTGGTTACTTGAGTTTCCCCTAGCTGAAGTTTCATATCATTTAAAG
CTGACCGCAAATCTTCGACTTCTTGAGTTTTGACTTGCTCAAGCTCCATCTGCATCAGTA
GGCAGATCAGATGCTTCACATAGAAAAGGGAAACATCAACAAAAGATCTACTATTGCAAG
TTCTGAGATGTGTACCCTCATTTGTTTCTCAAGCTCTAAGCATGAAGTGAGTTCTTCCAC
TTCCTTTTCTAGCTTAGTCTTAGCGTCTTGGAGGGCACCTGTTTCCTTAGCAGCCTGGAA
GACAAAAAAATATCTTAGGAAATCTCAGGATACATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 69-K107705-0022-692-D05C-8409
GenBank Accession LN512219 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K107705-0022-692-D05C-8409
CCTGAGATTTCCTAGATATTTTTTTGTCTTCCAGGCTGCTAAGGAAACCTTTGCCCTCCA
AGACGCTAAGACTAAGCTAGAAAAGGAAGTGGAAGAACTCACTTCATGCTTAGAGCTTGA
GAAACAAATGAGGGTACACATCTCAGAACTTGCAATAGTAGATCTTTTGTTGATGTTTCC
CTTTTCTATGTGAAGCATCTGATCTGCCTACTGATGCAGATGGAGCTTGAGCAAGTCAAA
ACTCAAGAAGTCGAAGATTTGCGGTCAGCTTTAAATGATATGAAACTTCAGCTAGGGGAA
ACTCAAGTAACCAAAAGTGAGGAGATTTTGAAGTTGCAGTCTGCTTTACAAGATATGCAA
CTAGAATTTGAAGAACTTGCTAAGGAGTTGGAGATGACAAATGATCTTGCTGCCGAAAAT
GAACAGCTCAAGGTAAGG

Last Updated on 06.12.2018 16:11