Sequences from confirmation amplicon


Confirmation sequences for line 692D06 and hit At1g10210 are shown.
Line ID 692D06
Locus At1g10210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3350338
Primers used for PCR GE52 - 8474, show primer details
   
Sequence Name 17-K100995-0022-692-D06C-8409
GenBank Accession LN493780 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K100995-0022-692-D06C-8409
ATTCGGACATGAGCCATTTACAATTGAATAGGCGAAAATGCAACCAACAGACCAAACATC
AATGGATGTTCCATAGTTGTCACAACAGAGGAGAAGCTCTGGGGCTCGGTACCAACGAGT
CACAACATATTCAGTCATGAACTGACCCTTGGTGTTGCTCGCACGCGCTAGTCCAAAATC
GCATATCTTTAAATCGCAGTTTGCGTTGACAAGAAGGTTACCAGGTTTCAAATCTCGGTG
CAGGATATTGGCTGAATGAATATACTTGAGCCCTCGAAGCAACTACACAAAAAGAACAAA
GCAAAGCAAAATTTCAGGAGAAAGCAAAGCTAGAACCAACAGTAAATGAACGGGATCAAC
CACACACCTGGAACAAGAAGTATTGGCAATGATCGTTACTAAGAACTTGAGAAGACTTGA
TAATCTGGTGGAGATCAGTGTCCATGAGCTCATAAACAAGATAAACATCCTTGAAGCTCA
TCTTATGAATTGGCATCATGACATCTTTCAAAGCAATGACATTCTCATGTCGAAGATGGC
GTAGAAGCTTGAGCTCCCGAAGAGTCCTCAACGCATCGATCCTATTCTCATAAACATTGT
GAATCTTCTTGATAGCAACTTTCTCGTTG
   
Sequence Name 17-K100999-0022-692-D06C-GE52
GenBank Accession LN493781 [GenBank]
Sequencing primer gene specific primer, GE52: 5'-GTTAACAGTGACACCAACGAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K100999-0022-692-D06C-GE52
ATGTTATGAGAATAGGATCGATGCGTTGAGGACTCTTCGGGAGCTCAAGCTTCTACGCCA
TCTTCGACATGAGAATGTCATTGCTTTGAAAGATGTCATGATGCCAATTCATAAGATGAG
CTTCAAGGATGTTTATCTTGTTTATGAGCTCATGGACACTGATCTCCACCAGATTATCAA
GTCTTCTCAAGTTCTTAGTAACGATCATTGCCAATACTTCTTGTTCCAGGTGTGTGGTTG
ATCCCGTTCATTTACTGTTGGTTCTAGCTTTGCTTTCTCCTGAAATTTTGCTTTGCTTTG
TTCTTTTTGTGTAGTTGCTTCGAGGGCTCAAGTATATTCATTCAGCCAATATCCTGCACC
GAGATTTGAAACCTGGTAACCTTCTTGTCAACGCAAACTGCGATTTAAAGATATGCGATT
TTGGACTAGCGCGTGCGAGCAACACCAAGGGTCAGTTCATGACTGAATATGTTGTGACTC
GTTGGTACCGAGCCCCAGAGCTTCTCCTCTGTTGTGACAACTATGGAACATCCATTGATG
TTTGGTCTGTTGGTTGCATTTTCGCCTATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11