Sequences from confirmation amplicon


Confirmation sequences for line 692E08 and hit At1g54115 are shown.
Line ID 692E08
Locus At1g54115 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20204100
Primers used for PCR 19nt - 8474, show primer details
   
Sequence Name 80-K114626-0022-692-E08C-19nt
GenBank Accession LN513980 [GenBank]
Sequencing primer gene specific primer, 19nt: 5'-TTGGACCCATTTTGTTCTCTATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K114626-0022-692-E08C-19nt
TGCTATATACTGTTTATAATATAATTTGGAAACCATTCACCAAGAAAATGATTTATTTGC
CTTCTTGTCCTTTCTTTTTTCTTTTTTCTTGTCGTTAACTTCTTGTTATTCTCTCTTGTC
GATTAGTTAAATCTAATTATTAGCTAAGCAATAGTAATCGGTATAAACTATTTTAATAAC
AACGTATGTTTCTTTGTTACGTAACATGAAAATTGAAAATCAGATAAACAATTAATTTTT
CCGATACGAATATCGCACTACCACGTGACGACGATGTACAACTACAAGTGGAAGCTAACG
AAACAAGTGGGGCAACACTATTGAGTTAAGCCGTGGCCCATTTATACTAAGGCCCATTAG
TCCCCATTTTCGAATACATAATCCATTTGTCCCAATCTACGATCGAGATCTACCACAACT
ATCTAGATCCAATGGCTCAGATTCATTCAGTTACATCAGCATAATAATACAAACAACATT
TACTTCAGAACCAGTGGGCTACGATATATTCGCCAGTGATCCAATGGCTATGATGCGCCG
CANACATCCGCACACATCTNCAGAACCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCANAAATGT
   
Sequence Name 80-K114625-0022-692-E08C-8409
GenBank Accession LN513979 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K114625-0022-692-E08C-8409
CTTACATTGAATATATCCTGGTTCTGAAGATGTGTGCGGATGTGTGCGGCGCATCATAGC
CATTGGATCACTGGCGAATATATCGTAGCCCACTGGTTCTGAAGTAAATGTTGTTTGTAT
TATTATGCTGATGTAACTGAATGAATCTGAGCCATTGGATCTAGATAGTTGTGGTAGATC
TCGATCGTAGATTGGGACAAATGGATTATGTATTCGAAAATGGGGACTAATGGGCCTTAG
TATAAATGGGCCACGGCTTAACTCAATAGTGTTGCCCCACTTGTTTCGTTAGCTTCCACT
TGTAGTTGTACATCGTCGTCACGTGGTAGTGCGATATTCGTATCGGAAAAATTAATTGTT
TATCTGATTTTCAATTTTCATGTTACGTAACAAAGAAACATACGTTGTTATTAAAATAGT
TTATACCGATTACTATTGCTTAGCTAATAATTAGATTTAACTAATCGACAAGAGAGAATA
ACAAGAAGTTAACGACAAGAAAAAAGAAAAAAGAAAGGACAAGAAGGCAAATAAATCATT
TTCTTGGTGAATGGTTTCCAAATTATATTATAAACAGTATATAGCATAAGTTATCATGTA
CCAAATGATTCTCTCGCATTAAAGTGAATAGAGAACAAAGGGGGGTCC

Last Updated on 06.12.2018 16:11