Sequences from confirmation amplicon


Confirmation sequences for line 692E09 and hit At3g02360 are shown.
Line ID 692E09
Locus At3g02360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:483877
Primers used for PCR BF05 - 8474, show primer details
   
Sequence Name 53-K028749-022-692-E09C-8409
GenBank Accession LN508041 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K028749-022-692-E09C-8409
CCTTTTCCAGAATTCTAGCTAACTCACCTAGCTTCAATCCCCACCCCTTTTCTAAGCTCT
TGGCACGGATAAAGTTCATGCCTTGTGCGTAGCTGCAGATTTTGGAAGCGTAAAGAGCCT
TCCTCACGTCATCGACTAGCTGTTTCTTGT
   
Sequence Name 53-K028749-022-692-E09C-BF05
GenBank Accession LN508042 [GenBank]
Sequencing primer gene specific primer, BF05: 5'-CGATCAGAAAGTTGACAAGAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K028749-022-692-E09C-BF05
AAATCTGCAGCACCACAAGGCATGAACCTTATCCGTGCCAAGAGCATAGAAAAGGGGTGG
GGATTGAAGCTAGGTGAGTTAGCTAGAATCTGGAAAGGAGGCTGCATCATCAGAGCAATC
TTCCTAGACAGGATCAAGCAAGCTTATGACAGGAACGCAGAGCTAGCTAACCTCTTGGTG
GATCCAGAGTTTGCGAAAGAGATAATCGAGAGACAGTCCGCGTGGAGAAGAGTCGTCTGC
TTAGCTATCAACTCGGGTATCAGCACACCGGGTATGTCTGCGAGTCTAGCCTACTTTGAT
TCTTACAGGAGAGAGAGATTGCCGGCAAATCTAGTCCAAGCACAGAGAGACTACTTTGGT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTC

Last Updated on 06.12.2018 16:11