Sequences from confirmation amplicon


Confirmation sequences for line 692H04 and hit At1g31120 are shown.
Line ID 692H04
Locus At1g31120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11104835
Primers used for PCR SD39 - 8474, show primer details
   
Sequence Name 27-K103556-0022-692-H04C-SD39
GenBank Accession LN498350 [GenBank]
Sequencing primer gene specific primer, SD39: 5'-ATTGAAGATGGCACTGTTTTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K103556-0022-692-H04C-SD39
CCTCTTGTGAACCTTGCCTCTCTCCTGGCTCGAACCACTNTGTTCCCCATTATATGCACC
ACTCCTGCGTCTCTGCACCCGTTTATGAACTCCACTTCGTCCCCTCCTCCGCTCATTTGG
CTCGACCCCGTCACTGTGTGACTTGTCCGTTTTGTTGTAGTGGGAGCTATAACCGACTCT
ATAGAATCAAATGTATCAAACGTCGACACGTTCCTAATCTCGTTCCCATTCCCGTTGACC
CCATCTCTGGACTGTCGTTGTTGGCTTCCGCAGACGCTGTAATCTTCGGAATCTGAGCAG
CCTTCCATCATCGATTCGAGGCGGAGGAAAAGGAAGAGGCTTTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 89-K103557-0022-692-H04C-8409
GenBank Accession LN514947 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K103557-0022-692-H04C-8409
CATTTACAATTGAAAGCCTCTTCCTTTTCCTCCGCCTCGAATCGACGATGGAAGGCTGCT
CAGATTCCGAAGATTACAGCGTCTGCGGAAGCCAACAACGACAGTCCAGAGATGGGGTCA
ACGGGAATGGGAACGAGATTAGGAACGTGTCGACGTTTGATACATTTGATTCTATAGAGT
CGGTTATAGCTCCCACTACAACAAAACGGACAAGTCACACAGTGACGGGGTCGAGCCAAA
TGAGCGGAGGAGGGGACGAAGTGGAGTTCATAAACGGGTGCAGAGACGCAGGAGTGGTGC
ATATAATGGGGAACACAGTGGTTCGAGCCAGGAGAGAGGCAAGGTTCTACAAGAGGATAG
CCATTGATTATGTTTATGCGTTTCTTAGGAAGATTTGTAGAGAAAACAGTGCCATCTTCA
A

Last Updated on 06.12.2018 16:11