Sequences from confirmation amplicon


Confirmation sequences for line 692H09 and hit At3g53260 are shown.
Line ID 692H09
Locus At3g53260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19746099
Primers used for PCR IF07 - 8474, show primer details
   
Sequence Name 20-K032518-022-692-H09C-8409
GenBank Accession LN495104 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K032518-022-692-H09C-8409
TAGTTTTGAGTATGCGTATCCACTTAGCCACTAATTTATTTATTTCTCTGATCACATTCC
TAATGAGTCCTAATTATTAATTATTATAATATAAGGTAAGATTTAATTAATTACCAAGAG
TTCTGTTTGTAGCGGTGCCGTTTTTGGTTCCCGGGAAATACCAACCG
   
Sequence Name 20-K032518-022-692-H09C-IF07
GenBank Accession LN495105 [GenBank]
Sequencing primer gene specific primer, IF07: 5'-AAAAACCACCAACAATTCAAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K032518-022-692-H09C-IF07
CATACAAGAGATCTGATCTCATTCACCTAAACACAACTTCTTGAAAACCAATGGATCAAA
TCGAAGCAATGTTGTGCGGCGGAGGAGAGAAGACAAAAGTGGCGGTTACTACGAAGACTT
TGGCAGATCCATTGAATTGGGGTTTAGCAGCGGATCAAATGAAAGGAAGTCATTTAGATG
AAGTGAAGAAGATGGTCGAAGAGTATCGTAGACCAGTCGTGAATCTTGGCGGAGAAACAC
TGACGATCGGACAAGTTGCTGCCATCTCCACCGTAGGAGGCAGCGTTAAGGTTGAGTTAG
CGGAGACTTCAAGAGCCGGTGTGAAAGCTAGCAGTGATTGGGTTATGGAGAGCATGAACA
AAGGTACTGACAGTTACGGAGTCACCACCGGCTTTGGTGCTACTTCTCACCGGAGAACCA
AAAACGGCACCGCATTACAAACAGAACTCATTAGGTAATTAATTAAATCCTATACCTTAT
ATTATATAACTAATTAATAATTAGAGAACTCATTAGGAATGTGATCAGAGAAATAAATAA
ATTAGTGGCTTAAGTGGATAACGCATACTCAAAACCTACGTGGTTATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTATNTCGAGTGTCTACTC
Primers used for PCR FQ56 - CR3S, show primer details
   
Sequence Name 33-K117832-0022-692-H09C-FQ56
GenBank Accession LN501170 [GenBank]
Sequencing primer gene specific primer, FQ56: 5'-CGATGTAAGAGAGAGGAACGAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K117832-0022-692-H09C-FQ56
TGACGGAGAGATGTTGTGGTTGAGGAGACTTGTAATCGCTTCGAGGATCTCGAATCGGAT
CCCGGAGTATCCTTGGAGAAGAGTGTTGACTCTGACGAGCATGGCGGCTCTTGTGGCGGA
TTGCGGCAGTGTGTGACATGTCTCCTTCGTGTTTCCGAATATTCCGGCGTTCAAAAATCT
GAAATCGGAAATAAACTTTAATTAATTAAAATAGACCCGACCCGACCGGTATCACCGACC
TGGATTTTGGTTTTAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATA
CATACTAAGGGTTTCTTATATGCTCAAACCAAANTCCAGGTCG
   
Sequence Name 05-K117831w-0022-692-H09C-CR3S
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117831w-0022-692-H09C-CR3S
CATAAAATTTCTAATTCCTAAACCAAAATCCAGGTCGGTGATACCGGTCGGGTCGGGTCT
ATTTTAATTAATTAAAGTTTATTTCCGATTTCAGATTTTTGAACGCCGGAATATTCGGAA
ACACGAAGGAGACATGTCACACACTGCCGCAATCCGCCACAAGAGCCGCCATGCTCGTCA
GAGTCAACACTCTTCTCCAAGGATACTCCGGGATCCGATTCGAGATCCTCGAAGCGATTA
CAAGTCTCCTCAACCACAACATCTCTCCGTCACTACCTCTCCGTGGAACCATTACCGCCT
CCGGCGATCTCGTTCCTCTCTCTAACATCG

Last Updated on 06.12.2018 16:11