Sequences from confirmation amplicon


Confirmation sequences for line 693D05 and hit At5g26000 are shown.
Line ID 693D05
Locus At5g26000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9080475
Primers used for PCR CL35 - 8474, show primer details
   
Sequence Name 21-K028745-022-693-D05C-8409
GenBank Accession LN495532 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K028745-022-693-D05C-8409
ATTTTAGTCGAATAAATCCACAGTTCGGTCCAAGAAATTCGAATTCAAAAAATTCGTCTA
CTTGGTACACGTTAGGAATTAGTCCCCCAGGTGATGAGGACTTTGAGAAGGCTACTGCCG
ATTACAAGCGGATTGATTATCTCTGTAGTCATCTCTGTTTCCTCAGTAAAGTCATCAAGT
GAGTGGATGATATATACTCTTCTTCTACTCTTGCTCTTTATATATACATAATGATTAACT
GTTCCTGTTTGTAG
   
Sequence Name 21-K028745-022-693-D05C-CL35
GenBank Accession LN495533 [GenBank]
Sequencing primer gene specific primer, CL35: 5'-TTGACATTCTTCTCCCTACAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K028745-022-693-D05C-CL35
AAGAGCAAGTAGTAGAAGAAGAAGTATATATCATCCACTCACTTGATGACTTTACTGAGG
AAACAGAGATGACTACAGAGATAATCAATCCGCTTGTAATCGGCAGTAGCCTTCTCAAAG
TCCTCATCACCTGGGGTACTAAATCCTAACATGTACCAAGTAGACAATATTTTGATTCAA
TTTCTTGGACCAACTGTGAATTAATACACTAAAAATGTATAGTTGAAAGAGAGTAGAACT
CACCATTCTCAGTGACATATATTAAAGGGTCACCGTAAGTGGTTTTGAAGTAATCCATTA
CGTAGTAAATGCCTTTTGGGTAGTAGTAACTGGCTGCATTGAACTGAGAAAATAAAGTAT
ATACATCAGAAAAAAGAAAAAATGAACGTTATTTTTGCTTAAACATAAATGTAAAAAACG
GATAATACATACCGGTGGACCAGGAGCATGACCAGTTGCATTTTTAGCTGTGAACACAAG
GGTGAAATAATTGATGGTCACTAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATT

Last Updated on 06.12.2018 16:11