Sequences from confirmation amplicon


Confirmation sequences for line 693D12 and hit At1g10900 are shown.
Line ID 693D12
Locus At1g10900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3634256
Primers used for PCR 26zb - 8474, show primer details
   
Sequence Name 10-K123399-0022-693-D12C-26zb
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 26zb: 5'-CTGTAGGAAAAATTACACCAGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K123399-0022-693-D12C-26zb
TAGACGCTAGAACAAAGATGTTCTTCCCTAGAGACGGCTCCAATTTTACCCCTCCACACA
AATCTGTCGACTTCTCCTGGAAAGACTATTGTCCCATGGTTTTCAGGTAAACCAGCTTTC
TTCTGGCTGATTACCAAAGAACTTTAGAATCAGAGTAGATAATCTTTGAATGTGTATATT
AGTTCTTGGATACTGTTCTCAAGTTAGATGTATTTTGTAAAAATTACGAACTACTTAGCT
CTGTGTGATCCCTCAGTTGTTCCTTTGGTCGGATAGAACACCTTTCTTAAATAAAGTAGT
TTAATTAACGATAAACCGTATAGATTGGGTAACATAGCAAGTAAGTTTTGTAGAGAGGTG
CATCTTTTTTACGCTTTGAGTTCCATGTTATCTGCTTTAGGCAGAAAGTTTTAACCAAAA
GGATTATTTTCTTCATAGACATGATTCTTAGTGCATCCTAGAAAATCTTACTTTTTGAGA
AAGCCAGAACTTTCGATTTGGGTTGTTTACCTCCCAAACGCAATGAGTGCAATCCATTAC
TTTTTTGGATTACGTAGCCGTGGGACGAAAAAATATGAAACAAGTGATTATGGAACCTGT
TCTTTGAATTGATAGACTGTGCGTGATTTTCAATGGAATGTTTCAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACA
GTTTATT
   
Sequence Name 01-K123398-0022-693-D12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K123398-0022-693-D12C-8409
CATTTCAATTGAATATATCCTGAAACATTCCATTGAAAATCACGCACAGTCTATCAATTC
AAAGAACAGGTTCCATAATCACTTGTTTCATATTTTTTCGTCCCACGGCTACGTAATCCA
AAAAAGTAATGGATTGCACTCATTGCGTTTGGGAGGTAAACAACCCAAATCGAAAGTTCT
GGCTTTCTCAAAAAGTAAGATTTTCTAGGATGCACTAAGAATCATGTCTATGAAGAAAAT
AATCCTTTTGGTTAAAACTTTCTGCCTAAAGCAGATAACATGGAACTCAAAGCGTAAAAA
AGATGCACCTCTCTACAAAACTTACTTGCTATGTTACCCAATCTATACGGTTTATCGTTA
ATTAAACTACTTTATTTAAGAAAGGTGTTCTATCCGACCAAAGGAACAACTGAGGGATCA
CACAGAGCTAAGTAGTTCGTAATTTTTACAAAATACATCTAACTTGAGAACAGTATCCAA
GAACTAATATACACATTCAAAGATTATCTACTCTGATTCTAAAGTTCTTTGGTAATCAGC
CAGAAGAAAGCTGGTTTACCTGAAAACCATGGGACAATAGTCTTTCCAGGAGAAGTCGAC
AGATTTGTGTGGAGGGGTAAAATTGGAGCCGTCTCTAGGGAAGAACATCTTTGTTCTAGC
GTTCTTACCAAAGTCAGAAGCACGCACTTCTCGCCTCGGCACTGGTGTAATTTTTCCTAC
AG

Last Updated on 06.12.2018 16:11