Sequences from confirmation amplicon


Confirmation sequences for line 693F07 and hit At1g28610 are shown.
Line ID 693F07
Locus At1g28610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10054999
Primers used for PCR 20sr - 8474, show primer details
   
Sequence Name 70-K116549-0022-693-F07C-20sr
GenBank Accession LN512450 [GenBank]
Sequencing primer gene specific primer, 20sr: 5'-GATTATCAGTTTCGGTGATTCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K116549-0022-693-F07C-20sr
ATCTTCCTGTGACCGCGTTTCTGCCGTATGGAGAAACCTTCTTCCACCACCCAACTGGCC
GTTCCTGCAACGGCCGTATCATCATCGACTTTATCGGTACTTCTCTTTCTATGTAACTTG
ACATGTAAGTAAGTGACTAGGAATGTAAATCTGATGAAGTATCGAATCATGATCTTATAA
TTGGTCTCAGTTGGAGTGTTTTAGACGTCTTCAATCTCTGAGAAGAATCCAAAAACACAA
AAAAAAACTGATAGTTGCGTTTGAACCTCTGTTTTCTTGTGTGGGCACAGCTTTTCCGAT
TTATAAAAATCTGGATTTTCTTGATTAGGGTTTCTTATGTAGACTCTTTCTCTGATTTGG
TTTTTCTTGATCTGCAAGTTAACAGTAGTATGAGACCTTAAGTGATTCTCCAATTTTCTA
TTTACAGCTGAATTCTTAGGGTTACCGCATGTGCCTCCTTTTTATGGATCTAAAAATGGA
AACTTTGAGAAAGGAGTTAATTTCGCTGTTTCTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 30-K116548-0022-693-F07C-8409
GenBank Accession LN499875 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K116548-0022-693-F07C-8409
TTTACATTGAAATATCCTGAGAAACAGCGAAATTAACTCCTTTCTCAAAGTTTCCATTTT
TAGATCCATAAAAAGGAGGCACATGCGGTAACCCTAAGAATTCAGCTGTAAATAGAAAAT
TGGAGAATCACTTAAGGTCTCATACTACTGTTAACTTGCAGATCAAGAAAAACCAAATCA
GAGAAAGAGTCTACATAAGAAACCCTAATCAAGAAAATCCAGATTTTTATAAATCGGAAA
AGCTGTGCCCACACAAGAAAACAGAGGTTCAAACGCAACTATCAGTTTTTTTTTGTGTTT
TTGGATTCTTCTCAGAGATTGAAGACGTCTAAAACACTCCAACTGAGACCAATTATAAGA
TCATGATTCGATACTTCATCAGATTTACATTCCTAGTCACTTACTTACATGTCAAGTTAC
ATAGAAAGAGAAGTACCGATAAAGTCGATGATGATACGGCCGTTGCAGGAACGGCCAGTT
GGGTGGTGGAAGAAGGTTTCTCCATACGGCAGAAACGCGGTCACAGGAAGATGGTTACGA
TCCGAGAGACCAACCAAGTTTCCTGTGTCGGTAATCGAATCA

Last Updated on 06.12.2018 16:11